大肠杆菌的groEL和dnaK基因以依赖htpR⁺的方式被紫外线照射和萘啶酸诱导。
groEL and dnaK genes of Escherichia coli are induced by UV irradiation and nalidixic acid in an htpR+-dependent fashion.
作者信息
Krueger J H, Walker G C
出版信息
Proc Natl Acad Sci U S A. 1984 Mar;81(5):1499-503. doi: 10.1073/pnas.81.5.1499.
Two proteins with molecular weights of 61,000 and 73,000 were found to be induced by UV light in Escherichia coli mutants in which the SOS responses are constitutively expressed. The induction of these proteins by UV light and nalidixic acid was shown to be independent of the recA+ lexA+ regulatory system. Analysis of these proteins by two-dimensional gel electrophoresis and comparison with the "heat-shock" proteins of E. coli revealed that the Mr 61,000 protein comigrated with the groEL gene product, that the Mr 73,000 protein comigrated with the dnaK gene product, and that other heat-shock proteins were also induced. The induction of groEL and dnaK by UV light and nalidixic acid is controlled by the htpR locus. The results suggest that the regulatory response of E. coli to agents such as UV light and nalidixic acid is more complex than previously thought.
在组成型表达SOS反应的大肠杆菌突变体中，发现两种分子量分别为61,000和73,000的蛋白质可被紫外线诱导。紫外线和萘啶酸对这些蛋白质的诱导作用显示出与recA⁺lexA⁺调节系统无关。通过二维凝胶电泳对这些蛋白质进行分析，并与大肠杆菌的“热休克”蛋白进行比较，结果表明，分子量为61,000的蛋白质与groEL基因产物迁移位置相同，分子量为73,000的蛋白质与dnaK基因产物迁移位置相同，并且还诱导了其他热休克蛋白。紫外线和萘啶酸对groEL和dnaK的诱导作用受htpR基因座控制。结果表明大肠杆菌对紫外线和萘啶酸等试剂的调节反应比以前认为的更为复杂。
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