CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamil Nadu, 608502, India.
Appl Biochem Biotechnol. 2014 Jan;172(1):9-20. doi: 10.1007/s12010-013-0499-1. Epub 2013 Sep 17.
Chitosan has attracted much attention as a biomedical material, owing to its unique biological activities. In this study, hepatoprotective effect of β-chitosan obtained from the gladius of squid Sepioteuthis lessoniana was studied against carbon tetrachloride (CCl4)-induced oxidative stress and liver injury in rats. The rats that received β-chitosan along with the administration of CCl4 showed significantly decreased plasma and tissue alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and total cholesterol, triglyceride (TG) and free fatty acid (FFA) contents, whereas the treatment with β-chitosan alone markedly increased rat hepatic and circulatory superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) and reduced glutathione (GSH) levels and decreased the malondialdehyde level. Histopathological observations recommended the marked hepatoprotective effect of β-chitosan. The CCl4-induced alterations on circulatory and hepatic antioxidant defence system were normalised by β-chitosan, and it could be concluded that the hepatoprotective effect of chitosan may be due to its antioxidant and antilipidemic property. Therefore, β-chitosan could be considered as antihepatotoxic agent.
壳聚糖因其独特的生物学活性而引起了人们的广泛关注，作为一种生物医学材料。在这项研究中，研究了来自鱿鱼 Sepioteuthis lessoniana 的鱼鳍的β-壳聚糖对四氯化碳（CCl4）诱导的大鼠氧化应激和肝损伤的保护作用。与 CCl4 一起接受β-壳聚糖治疗的大鼠的血浆和组织丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）活性以及总胆固醇，甘油三酯（TG）和游离脂肪酸（FFA）含量明显降低，而单独用β-壳聚糖治疗则显著增加了大鼠肝和循环中超氧化物歧化酶（SOD），过氧化氢酶和谷胱甘肽过氧化物酶（GPx）和还原型谷胱甘肽（GSH）水平，并降低了丙二醛水平。组织病理学观察表明β-壳聚糖具有明显的保肝作用。β-壳聚糖使 CCl4 引起的循环和肝抗氧化防御系统的改变正常化，可以得出结论，壳聚糖的保肝作用可能是由于其抗氧化和抗脂质作用。因此，可以将β-壳聚糖视为抗肝毒素剂。
