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杜氏枪乌贼内壳壳聚糖纳米聚集体的合成与表征

Synthesis and Characterization of Chitosan Nanoaggregates from Gladius of Uroteuthis duvauceli.

作者信息

Anusha J R, Fleming Albin T

机构信息

Department of Advanced Zoology and Biotechnology, Loyola College, Chennai, Tamil Nadu 600 034, India.

出版信息

Int J Biomater. 2016;2016:5379424. doi: 10.1155/2016/5379424. Epub 2016 Feb 10.

DOI:10.1155/2016/5379424
PMID:26977152
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4764718/
Abstract

We report the synthesis, characterization, and biological properties of chitosan nanoaggregates from gladius of squid, Uroteuthis duvauceli. β-Chitin extracted from gladius was deacetylated to chitosan and further reduced to nanosize using ionic gelation process. The morphology and occurrence of chitosan nanoaggregates (CSNA) were observed using transmission electron microscopy (TEM). The degree of deacetylation (DD%) calculated from Fourier transform infrared (FTIR) spectrum showed high value (~94 ± 1.25%) for chitosan. The CSNA depicts low molecular weight, stable positive zeta potential, and less ash and moisture content with high water and fat binding capacity. The antimicrobial activity was tested against pathogenic microorganisms, which depicted significant rate of inhibition against Staphylococcus aureus and Escherichia coli due to high cellular uptake. The antioxidant analysis for CSNA demonstrated high reducing power and scavenging activity towards superoxide radicals compared with the commercially available chitosan. Furthermore, nanoaggregates exhibited low cytotoxic behavior in biological in vitro tests performed using cervical cancer cell line. These results indicate that chitosan nanoaggregates synthesized from waste gladius will be highly efficient and safe candidate for biological applications as food packing film, drug carrier, and tissue engineering.

摘要

我们报道了来自杜氏枪乌贼（Uroteuthis duvauceli）内壳的壳聚糖纳米聚集体的合成、表征及生物学特性。从内壳中提取的β-几丁质经脱乙酰化制成壳聚糖，并通过离子凝胶化过程进一步缩小至纳米尺寸。使用透射电子显微镜（TEM）观察壳聚糖纳米聚集体（CSNA）的形态和出现情况。由傅里叶变换红外（FTIR）光谱计算得出的脱乙酰度（DD%）显示壳聚糖具有较高的值（约94±1.25%）。CSNA呈现出低分子量、稳定的正ζ电位、较低的灰分和水分含量以及较高的水和脂肪结合能力。针对致病微生物测试了其抗菌活性，由于高细胞摄取率，其对金黄色葡萄球菌和大肠杆菌呈现出显著的抑制率。与市售壳聚糖相比，CSNA的抗氧化分析表明其具有较高的还原能力和对超氧自由基的清除活性。此外，在使用宫颈癌细胞系进行的体外生物学测试中，纳米聚集体表现出低细胞毒性行为。这些结果表明，由废弃内壳合成的壳聚糖纳米聚集体作为食品包装薄膜、药物载体和组织工程的生物应用将是高效且安全的候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6be5/4764718/622d260c4fdb/IJBM2016-5379424.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6be5/4764718/ae276c093817/IJBM2016-5379424.sch.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6be5/4764718/6fed7c074b40/IJBM2016-5379424.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6be5/4764718/f3cd9b9b8421/IJBM2016-5379424.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6be5/4764718/4ec776d43c37/IJBM2016-5379424.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6be5/4764718/3bc6a82bd42b/IJBM2016-5379424.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6be5/4764718/622d260c4fdb/IJBM2016-5379424.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6be5/4764718/ae276c093817/IJBM2016-5379424.sch.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6be5/4764718/6fed7c074b40/IJBM2016-5379424.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6be5/4764718/f3cd9b9b8421/IJBM2016-5379424.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6be5/4764718/4ec776d43c37/IJBM2016-5379424.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6be5/4764718/3bc6a82bd42b/IJBM2016-5379424.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6be5/4764718/622d260c4fdb/IJBM2016-5379424.005.jpg

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