Tran Hong Hanh Thi, Nguyen Minh Chau, Le Hoang Tram, Nguyen Thi Luyen, Pham Thanh Binh, Chau Van Minh, Nguyen Hoai Nam, Nguyen Tien Dat
Institute of Marine Biochemistry, Vietnam Academy of Science and Technology , 18-Hoang Quoc Viet, Cau Giay, Hanoi , Vietnam and.
Pharm Biol. 2014 Jan;52(1):74-7. doi: 10.3109/13880209.2013.814692. Epub 2013 Sep 18.
A methanol extract of Cyperus rotundus L. (Cyperaceae) rhizomes showed inhibitory activity against α-glucosidase and α-amylase, two enzymes involve in carbohydrate digestion.
Identification of compounds from C. rotundus rhizomes responsible for the inhibition of α-glucosidase and α-amylase.
Compounds were identified by a phytochemical investigation using combined chromatographic and spectroscopic methods. α-glucosidase and α-amylase inhibitory activities were evaluated by in vitro enzyme inhibition assays.
A new (2RS,3SR)-3,4',5,6,7,8-hexahydroxyflavane (1), together with three known stilbene dimers cassigarol E (2), scirpusin A (3) and B (4) were isolated. Compound 2 inhibited both α-glucosidase and α-amylase activities while the flavane 1 only showed effect on α-amylase, and compounds 3 and 4 were active on α-glucosidase. All four compounds showed significant 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity.
The inhibitory activities against α-amylase and α-glucosidase of the C. rotundus rhizomes were reported for the first time. Stilbene dimers are considered as potent inhibitors of α-glucosidase and promising antihyperglycemic agents.
The isolated compounds may contribute to the antidiabetic property of C. rotundus.
香附子（莎草科）根茎的甲醇提取物对α-葡萄糖苷酶和α-淀粉酶具有抑制活性，这两种酶参与碳水化合物的消化过程。
鉴定香附子根茎中负责抑制α-葡萄糖苷酶和α-淀粉酶的化合物。
通过联合色谱和光谱方法进行植物化学研究来鉴定化合物。通过体外酶抑制试验评估α-葡萄糖苷酶和α-淀粉酶的抑制活性。
分离得到一种新的(2RS,3SR)-3,4',5,6,7,8-六羟基黄烷（1），以及三种已知的芪二聚体，即卡西加洛尔E（2）、荸荠素A（3）和B（4）。化合物2同时抑制α-葡萄糖苷酶和α-淀粉酶的活性，而黄烷1仅对α-淀粉酶有作用，化合物3和4对α-葡萄糖苷酶有活性。所有四种化合物都表现出显著的2,2-二苯基-1-苦基肼基（DPPH）清除活性。
首次报道了香附子根茎对α-淀粉酶和α-葡萄糖苷酶的抑制活性。芪二聚体被认为是α-葡萄糖苷酶的有效抑制剂和有前景的抗高血糖药物。
分离得到的化合物可能有助于香附子的抗糖尿病特性。
