L.对关键碳水化合物代谢酶比活性的调节作用动力学
KINETICS OF MODULATORY ROLE OF L. ON THE SPECIFIC ACTIVITY OF KEY CARBOHYDRATE METABOLIZING ENZYMES.
作者信息
Sabiu Saheed, Ajani Emmanuel Oladipo, Sunmonu Taofik Olatunde, Ashafa Anofi Omotayo Tom
机构信息
Phytomedicine, Food Factors and Toxicology Research Laboratory, Biochemistry Unit, Department of Biosciences and Biotechnology, Kwara State University, Malete, Nigeria.
Phytomedicine and Phytopharmacology Research Group, Plant Sciences Department, University of the Free State, Qwaqwa campus, Phuthaditjhaba 9866, South Africa.
出版信息
Afr J Tradit Complement Altern Med. 2017 Jun 5;14(4):46-53. doi: 10.21010/ajtcam.v14i4.6. eCollection 2017.
BACKGROUND
The continuous search for new lead compounds as viable inhibitors of specific enzymes linked to carbohydrate metabolism has intensified. L. is one of the therapeutically implicated botanicals against several degenerative diseases including diabetes mellitus.
MATERIALS AND METHODS
This study evaluated the antioxidant and mechanism(s) of inhibitory potential of aqueous extract of on α-amylase and α-glucosidase . The extract was investigated for its radical scavenging and hypoglycaemic potentials using standard experimental procedures. Lineweaver-Burke plot was used to predict the manner in which the enzymes were inhibited.
RESULTS
The data obtained revealed that the extract moderately and potently inhibited the specific activities of -amylase and -glucosidase, respectively. The inhibition was concentration-related with respective IC values of 5.19 and 0.78 mg/mL relative to that of the control (3.72 and 3.55 mg/mL). The extract also significantly scavenged free radicals and the effects elicited could be ascribed to its phytoconstituents.
CONCLUSION
The respective competitive and non-competitive mode of action of the extract is due to its inhibitory potentials on the activities of -amylase and -glucosidase. Going forward, in addition to completely characterize the exact compound(s) responsible for the elicited activity in this study, pertinent attention will be given to the evaluation of the identified constituents.
背景
对于作为与碳水化合物代谢相关的特定酶的可行抑制剂的新先导化合物的持续探索已经加强。L.是与包括糖尿病在内的几种退行性疾病相关的具有治疗意义的植物药之一。
材料与方法
本研究评估了L.水提取物对α-淀粉酶和α-葡萄糖苷酶的抗氧化及抑制潜力机制。使用标准实验程序研究了该提取物的自由基清除和降血糖潜力。采用Lineweaver-Burke图来预测酶的抑制方式。
结果
获得的数据表明，该提取物分别适度且有效地抑制了α-淀粉酶和α-葡萄糖苷酶的比活性。抑制作用与浓度相关，相对于对照（3.72和3.55 mg/mL），各自的IC值分别为5.19和0.78 mg/mL。该提取物还显著清除自由基，其引发的作用可归因于其植物成分。
结论
该提取物各自的竞争性和非竞争性作用模式归因于其对α-淀粉酶和α-葡萄糖苷酶活性的抑制潜力。展望未来，除了完全表征本研究中引发活性的确切化合物外，还将对鉴定出的成分进行相关评估。
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