Russo Erica, Nitschké Maximilian, Halin Cornelia
Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology , ETH Zurich, Switzerland .
Lymphat Res Biol. 2013 Sep;11(3):172-82. doi: 10.1089/lrb.2013.0008.
Afferent lymphatic vessels fulfill essential immune functions by transporting leukocytes and lymph-borne antigen to draining lymph nodes (dLNs). An important cell type migrating through lymphatic vessels are dendritic cells (DCs). DCs reside in peripheral tissues like the skin, where they take up antigen and transport it via the lymphatic vascular network to dLNs for subsequent presentation to T cells. As such, DCs play a key role in the induction of adaptive immune responses during infection and vaccination, but also for the maintenance of tolerance. Although the migratory pattern of DCs has been known for long time, interactions between DCs and lymphatic vessels are only now starting to be unraveled at the cellular level. In particular, new tools for visualizing lymphatic vessels in combination with time-lapse microscopy have recently generated valuable insights into the process of DC migration to dLNs. In this review we summarize and discuss current approaches for visualizing DCs and lymphatic vessels in tissues for imaging applications. Furthermore, we review the current state of knowledge about DC migration towards, into and within lymphatic vessels, particularly focusing on the cellular interactions that take place between DCs and the lymphatic endothelium.
输入淋巴管通过将白细胞和淋巴携带的抗原运输到引流淋巴结（dLN）来履行重要的免疫功能。一种通过淋巴管迁移的重要细胞类型是树突状细胞（DC）。DC存在于皮肤等外周组织中，在那里它们摄取抗原，并通过淋巴管网络将其运输到dLN，以便随后呈递给T细胞。因此，DC在感染和疫苗接种期间适应性免疫反应的诱导中起着关键作用，而且在维持耐受性方面也起着关键作用。尽管DC的迁移模式早已为人所知，但DC与淋巴管之间的相互作用直到现在才开始在细胞水平上得到揭示。特别是，用于可视化淋巴管的新工具与延时显微镜相结合，最近为DC迁移到dLN的过程提供了有价值的见解。在这篇综述中，我们总结并讨论了用于组织成像应用中可视化DC和淋巴管的当前方法。此外，我们回顾了目前关于DC向淋巴管迁移、进入淋巴管以及在淋巴管内迁移的知识状态，特别关注DC与淋巴管内皮之间发生的细胞相互作用。
