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高效糖核苷酸的再生用于大规模酶促合成 Globo H 和 SSEA4。

Effective sugar nucleotide regeneration for the large-scale enzymatic synthesis of Globo H and SSEA4.

机构信息

Genomics Research Center, Academia Sinica , No. 128, Section 2, Academia Road, Taipei 115, Taiwan.

出版信息

J Am Chem Soc. 2013 Oct 2;135(39):14831-9. doi: 10.1021/ja4075584. Epub 2013 Sep 17.

DOI:10.1021/ja4075584
PMID:24044869
Abstract

We report here the development of chemoenzymatic methods for the large-scale synthesis of cancer-associated antigens globopentaose (Gb5), fucosyl-Gb5 (Globo H), and sialyl-Gb5 (SSEA4) by using overexpressed glycosyltransferases coupled with effective regeneration of sugar nucleotides, including UDP-Gal, UDP-GalNAc, GDP-Fuc, and CMP-Neu5Ac. The enzymes used in the synthesis were first identified from different species through comparative studies and then overexpressed in E. coli and isolated for synthesis. These methods provide multigram quantities of products in high yield with only two or three purification steps and are suitable for the evaluation and development of cancer vaccines and therapeutics.

摘要

我们在此报告了通过过表达糖基转移酶并结合有效的核苷酸糖再生，包括 UDP-Gal、UDP-GalNAc、GDP-Fuc 和 CMP-Neu5Ac，来大规模合成癌症相关抗原 globopentaose (Gb5)、岩藻糖基-Gb5 (Globo H) 和唾液酸化-Gb5 (SSEA4) 的化学酶法。用于合成的酶首先通过比较研究从不同物种中鉴定出来，然后在大肠杆菌中过表达并分离用于合成。这些方法仅需两到三个纯化步骤，即可提供多克数量的高产量产物，适用于癌症疫苗和疗法的评估和开发。

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