Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
Bioresour Technol. 2013 Nov;148:144-8. doi: 10.1016/j.biortech.2013.08.052. Epub 2013 Aug 14.
The bacterium isolated from sea sludge Paracoccus versutus LYM was characterized with the ability of aerobic denitrification. Strain LYM performs perfect activity in aerobically converting over 95% NO3(-)-N (approximate 400mg L(-1)) to gaseous products via nitrite with maximum reduction rate 33 mg NO3(-)-N L(-1) h(-1). Besides characteristic of aerobic denitrification, strain LYM was confirmed in terms of the ability to be heterotrophic nitrification and aerobic denitrification (HNAD) with few accumulations of intermediates. After the nitrogen balance and enzyme assays, the putative nitrogen pathway of HNAD could be NH4(+) → NH2OH → NO2(-)→ NO3(-), then NO3(-) was denitrified to gaseous products via nitrite. N2 was sole denitrification product without any detection of N2O by gas chromatography. Strain LYM could also simultaneously remove ammonium and additional nitrate. Meanwhile, the accumulated nitrite had inhibitory effect on ammonium reduction rate.
从海泥中分离得到的小球菌 Paracoccus versutus LYM 具有好氧反硝化能力。LYM 菌株在好氧条件下可将超过 95%的 NO3(-)-N（约 400mg/L）通过亚硝酸盐转化为气态产物，最大还原速率为 33mg NO3(-)-N/L/h。除了具有好氧反硝化特性外，LYM 菌株还被证实具有异养硝化和好氧反硝化（HNAD）的能力，中间产物积累较少。通过氮平衡和酶测定，HNAD 的可能氮途径可以是 NH4(+)→NH2OH→NO2(-)→NO3(-)，然后通过亚硝酸盐将 NO3(-) 反硝化生成气态产物。通过气相色谱法没有检测到 N2O，N2 是唯一的反硝化产物。LYM 菌株还可以同时去除铵盐和额外的硝酸盐。同时，积累的亚硝酸盐对铵盐还原速率有抑制作用。
