Abdullah Murdani, Rani A Aziz, Sudoyo Aru W, Makmun Dadang, Handjari Diah R, Hernowo Bethy S
Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia - Cipto Mangunkusumo Hospital, Jakarta, Indonesia.
Acta Med Indones. 2013 Jul;45(3):187-92.
to evaluate the expression of NF-kB and COX2 in native Indonesians with sporadic colorectal cancer (CRC).
we conducted a matched-pair case-control study by acquiring both CRC and tumor-adjacent normal tissues from the same subjects. CRC patients who underwent surgery at Cipto Mangunkusumo Hospital, Jakarta, or Hasan Sadikin Hospital, Bandung, were enrolled in the study. The specimens were immunohistologically stained with antibody directed against p65 (RelA) to assess NF-kB expression and against human COX2 protein to assess COX2 expression.
sixty-seven specimens consisting of both CRC and tumor-adjacent normal tissues were analyzed. COX2 expression was positive in 39 CRC tissues (58.2%), but in only 19 tumor-adjacent normal tissues (28.4%; p=0.0002). NF-kB expression was positive in 47 CRC tissues (70.1%), but in only 27 tumor-adjacent normal tissues (40.3%; p<0.0001).
inflammation plays a role in the carcinogenesis of sporadic CRC in native Indonesians. This support potential use of nonsteroidal anti-inflammatory drugs as chemopreventive agents for CRC.
评估核因子-κB（NF-κB）和环氧化酶2（COX2）在印度尼西亚散发性结直肠癌（CRC）患者中的表达情况。
我们开展了一项配对病例对照研究，从同一受试者身上获取CRC组织及肿瘤旁正常组织。纳入了在雅加达的芝多·曼古库苏莫医院或万隆的哈桑·萨迪金医院接受手术的CRC患者。对标本进行免疫组织化学染色，使用针对p65（RelA）的抗体评估NF-κB表达，使用针对人COX2蛋白的抗体评估COX2表达。
共分析了67份包含CRC组织及肿瘤旁正常组织的标本。COX2在39份CRC组织中呈阳性表达（58.2%），而在仅19份肿瘤旁正常组织中呈阳性表达（28.4%；p = 0.0002）。NF-κB在47份CRC组织中呈阳性表达（70.1%），而在仅27份肿瘤旁正常组织中呈阳性表达（40.3%；p < 0.0001）。
炎症在印度尼西亚散发性CRC的致癌过程中起作用。这支持将非甾体类抗炎药作为CRC的化学预防剂的潜在用途。
