Alamro Reem Abdullah, Mustafa Mohammad, Al-Asmari Abdulrahman K
Deparment of Gastroenterology, Prince Sultan Military Medical City, Riyadh, Saudi Arabia.
Research Centre, Prince Sultan Military Medical City, Riyadh, Saudi Arabia,
J Inflamm Res. 2018 Sep 24;11:351-357. doi: 10.2147/JIR.S155507. eCollection 2018.
The present study planned to investigate the changes in the mRNA expression of inflammatory genes and their association with colorectal cancer (CRC). Our findings could be useful for noninvasive early screening of CRC patients.
Venous blood of 20 CRC cases and 15 healthy controls was collected. The mRNA expression of COX-2, TNF-α, NF-κB and IL-6 genes was carried out by using real-time polymerase chain reaction. Relative quantification was done to find out the fold change of these genes.
The mean age of cases and controls was 55 and 50 years, respectively. The ΔCt of COX-2, TNF-α, NF-κB and IL-6 genes was significantly ( < 0.05) lower in cases as compared to controls. Subsequently, the mRNA expression of these genes was, respectively, 3.56-, 3.4-, 1.71- and 3.86-fold higher in CRC cases as compared to controls. Positive correlation of ΔCt of COX-2 was found with ΔCt of TNF-α ( = 0.461, = 0.041) and NF-κB ( = 0.536, = 0.015) in CRC cases. The mRNA expression of COX-2 was significantly lower in T2 stage, while mRNA expression of NF-κB was significantly lower in both T2 and T3 stages of CRC as compared to T4 stage.
The increased mRNA expression of COX-2 along with the high mRNA expression of TNF-α, NF-κB and IL-6 genes may be associative risk factors for CRC. COX-2 and NF-κB genes were more expressed in advanced stages of CRC indicating their role in tumor progression. Our findings support the possible role of blood biomarker in the screening of CRC patients in the early stages.
本研究旨在调查炎症基因的mRNA表达变化及其与结直肠癌（CRC）的关联。我们的研究结果可能有助于CRC患者的无创早期筛查。
收集20例CRC患者和15名健康对照者的静脉血。采用实时聚合酶链反应检测COX-2、TNF-α、NF-κB和IL-6基因的mRNA表达。进行相对定量以找出这些基因的倍数变化。
病例组和对照组的平均年龄分别为55岁和50岁。与对照组相比，病例组中COX-2、TNF-α、NF-κB和IL-6基因的ΔCt显著更低（<0.05）。随后，与对照组相比，CRC病例中这些基因的mRNA表达分别高3.56倍、3.4倍、1.71倍和3.86倍。在CRC病例中，发现COX-2的ΔCt与TNF-α的ΔCt（r = 0.461，P = 0.041）和NF-κB的ΔCt（r = 0.536，P = 0.015）呈正相关。与T4期相比，COX-2的mRNA表达在T2期显著降低，而NF-κB的mRNA表达在CRC的T2和T3期均显著低于T4期。
COX-2的mRNA表达增加以及TNF-α、NF-κB和IL-6基因的高mRNA表达可能是CRC的相关危险因素。COX-2和NF-κB基因在CRC晚期表达更高，表明它们在肿瘤进展中的作用。我们的研究结果支持血液生物标志物在早期CRC患者筛查中的可能作用。
