Lavoie J P, Madigan J E, Cullor J S, Powell W E
Veterinary Medical Teaching Hospital, Department of Medicine, University of California, Davis 95616.
Equine Vet J. 1990 Jan;22(1):23-9. doi: 10.1111/j.2042-3306.1990.tb04198.x.
The purpose of this study was to evaluate the effects of experimentally induced sublethal endotoxaemia in equine neonates. Four foals, between two and five days of age, were infused intravenously with 0.5 microgram/kg bodyweight of Salmonella typhimurium endotoxin (LPS) over a 5 h period. A four-day-old and a five-day-old foal, similarly infused with sterile isotonic saline, served as controls. Clinical signs were monitored, blood samples obtained for evaluation of selected haematological and biochemical parameters; and haemodynamic parameters were recorded hourly during the infusion, as well as 6 and 24 h post infusion. Depression, anorexia, increased rectal temperature, leucopenia followed by leucocytosis, hypoglycaemia, increased prothrombin time, partial thromboplastin time (APTT), pulmonary artery pressure, pulmonary artery wedge pressure, right atrial pressure, pulmonary and systemic vascular resistance and mild hypoxaemia were consistent findings in the foals receiving endotoxin. There was marked variation over time in the above parameters, during the infusion. Shock was not induced, and the foals appeared to be healthy shortly after the infusion was discontinued. The return to baseline values of body temperature (3 of 4 foals), APTT (1 of 4 foals) and neutrophil count (2 of 4 foals), during endotoxin infusion, suggests induction of early tolerance. The control foals remained alert and the temperature, prothrombin time and fibrinogen remained stable during the study. Hyperglycaemia, transient increased APTT and variations in selected haemodynamic parameters were recorded in the control foals during the infusion.
本研究的目的是评估实验诱导的亚致死性内毒素血症对马驹新生儿的影响。四匹年龄在2至5天之间的马驹在5小时内静脉输注0.5微克/千克体重的鼠伤寒沙门氏菌内毒素（LPS）。一匹4日龄和一匹5日龄的马驹同样输注无菌等渗盐水作为对照。监测临床症状，采集血样以评估选定的血液学和生化参数；在输注期间以及输注后6小时和24小时每小时记录血流动力学参数。在接受内毒素的马驹中，抑郁、厌食、直肠温度升高、白细胞减少后白细胞增多、低血糖、凝血酶原时间延长、部分凝血活酶时间（APTT）延长、肺动脉压升高、肺动脉楔压升高、右心房压升高、肺血管阻力和全身血管阻力升高以及轻度低氧血症是一致的发现。在输注期间，上述参数随时间有明显变化。未诱发休克，输注停止后不久马驹看起来健康。在内毒素输注期间，体温（4匹中的3匹）、APTT（4匹中的1匹）和中性粒细胞计数（4匹中的2匹）恢复到基线值，提示诱导了早期耐受性。对照马驹在研究期间保持警觉，体温、凝血酶原时间和纤维蛋白原保持稳定。在输注期间，对照马驹记录到高血糖、APTT短暂升高以及选定血流动力学参数的变化。
