囊性纤维化患者呼吸道样本中非结核分枝杆菌培养的采样与净化方法
Sampling and decontamination method for culture of nontuberculous mycobacteria in respiratory samples of cystic fibrosis patients.
作者信息
De Bel Annelies, De Geyter Deborah, De Schutter Iris, Mouton Christine, Wellemans Isabelle, Hanssens Laurence, Schelstraete Petra, Malfroot Anne, Pierard Denis
机构信息
Department of Microbiology and Infection Control.
出版信息
J Clin Microbiol. 2013 Dec;51(12):4204-6. doi: 10.1128/JCM.02035-13. Epub 2013 Sep 18.
Abstract
We confirmed that chlorhexidine decontamination yielded more nontuberculous mycobacteria than did the N-acetyl-l-cysteine-NaOH-oxalic acid procedure from respiratory samples of cystic fibrosis patients on solid cultures. However, this improved recovery is mostly balanced if the latter is combined with liquid culture. Furthermore, none of the 145 cough swabs, used to sample young children, cultured positive, suggesting that swabs are low-quality samples.
摘要
我们证实，在固体培养基上，对于囊性纤维化患者的呼吸道样本，洗必泰去污法比N-乙酰-L-半胱氨酸-氢氧化钠-草酸法产生更多的非结核分枝杆菌。然而，如果将后者与液体培养相结合，这种回收率的提高大多会被抵消。此外，用于采集幼儿样本的145份咳嗽拭子均未培养出阳性结果，这表明拭子是质量较差的样本。
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本文引用的文献
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Cough swabs should not be used to isolate non-tuberculous mycobacteria in children with cystic fibrosis.咳嗽拭子不应用于隔离囊性纤维化患儿中的非结核分枝杆菌。
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Value of the chlorhexidine decontamination method for recovery of nontuberculous mycobacteria from sputum samples of patients with cystic fibrosis.洗必泰去污法对从囊性纤维化患者痰液样本中回收非结核分枝杆菌的价值。
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