Amanfu W, Weng C N, Ross R F, Barnes H J
Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
Am J Vet Res. 1984 Jul;45(7):1349-52.
An improved direct fluorescent antibody test was evaluated for specificity and efficacy in diagnosing mycoplasmal pneumonia of swine. A sequential study was carried out in which pigs inoculated with a pneumonic lung suspension containing Mycoplasma hyopneumoniae strain 11 were euthanatized at postinoculation (PI) weeks 2 to 12. Fluorescent coating of M hyopneumoniae was detected primarily on bronchial and bronchiolar epithelial surfaces of lungs with gross lesions of pneumonia. Fluorescence was observed to be most intense at PI weeks 4 to 6, with a tendency to decrease in intensity from PI weeks 8 to 12. This indicated that there may be a decrease in number of M hyopneumoniae cells in the more advanced stages of the disease. The use of a counterstain (chelated azo-dye) provided an excellent color contrast and permitted making unambiguous interpretation of results.
对一种改进的直接荧光抗体试验在诊断猪支原体肺炎中的特异性和有效性进行了评估。进行了一项序贯研究，在接种含有猪肺炎支原体11型的肺炎肺悬液的猪中，在接种后（PI）第2至12周实施安乐死。猪肺炎支原体的荧光包被主要在有明显肺炎病变的肺的支气管和细支气管上皮表面检测到。观察到荧光在PI第4至6周时最强，从PI第8至12周强度有下降趋势。这表明在疾病的更晚期猪肺炎支原体细胞数量可能减少。使用复染剂（螯合偶氮染料）提供了出色的颜色对比，并允许对结果进行明确的解释。
