Debey M C, Roth J A, Ross R F
National Animal Disease Center, Ames, IA 50010.
Vet Res Commun. 1993;17(4):249-57. doi: 10.1007/BF01839215.
Neutrophils isolated from the peripheral blood of pigs free of infection with Mycoplasma hyopneumoniae were loaded with a fluorescent indicator (Fura-2) for detection of cytosolic free calcium concentration. The kinetics of the intracellular calcium flux were examined after incubation with or without a pathogenic or a non-pathogenic strain of M. hyopneumoniae. The basal intracellular calcium concentration was not altered by incubation with M. hyopneumoniae. However, the relative increase in cytoplasmic calcium concentration caused by the addition of opsonized zymosan was significantly (p < 0.05) higher in neutrophils incubated with M. hyopneumoniae as compared to neutrophils not incubated with M. hyopneumoniae. Additionally, after zymosan stimulation, the intracellular calcium concentration was greater in neutrophils incubated with a pathogenic strain of M. hyopneumoniae than in those incubated with a non-pathogenic strain. This suggests that M. hyopneumoniae alters the signal transduction mechanisms in neutrophils and that this alteration may be related to virulence.
从无猪肺炎支原体感染的猪外周血中分离出中性粒细胞，用荧光指示剂（Fura-2）加载以检测胞质游离钙浓度。在用致病性或非致病性猪肺炎支原体菌株孵育后，检测细胞内钙通量的动力学。与猪肺炎支原体孵育不会改变基础细胞内钙浓度。然而，与未与猪肺炎支原体孵育的中性粒细胞相比，与猪肺炎支原体孵育的中性粒细胞中，添加调理酵母聚糖引起的细胞质钙浓度相对增加显著更高（p < 0.05）。此外，在酵母聚糖刺激后，与致病性猪肺炎支原体菌株孵育的中性粒细胞中的细胞内钙浓度高于与非致病性菌株孵育的中性粒细胞。这表明猪肺炎支原体改变了中性粒细胞中的信号转导机制，并且这种改变可能与毒力有关。
