Sands J A, Lowlicht R A, Cadden S C, Haneman J
Can J Microbiol. 1975 Aug;21(8):1287-90. doi: 10.1139/m75-194.
The effects of known membrane-perturbing agents (pH, Na+, Ca2+, and a small lipid-soluble molecule) on the enveloped bacteriophage phi 6 host cell system were investigated at the levels of cellular growth, virus assembly and stability, and the physical and chemical properties of host cell membranes. Spin-label probes of cellular membranes indicate that growth in high levels of Na+ or the small spherical hydrophobic molecule adamantanone results in membranes having increased "fluidity," while growth in high levels of Ca2+ results in slightly greater rigidity of the membranes. In addition, the phospholipid composition of the cellular membranes is dependent on the NaCl concentration in the growth medium. None of these membrane alterations, however, prevent the production of infectious phi 6 virus particles.
研究了已知的膜扰动剂（pH值、Na⁺、Ca²⁺和一种小的脂溶性分子）对包膜噬菌体phi 6宿主细胞系统在细胞生长、病毒组装与稳定性以及宿主细胞膜的物理和化学性质等层面的影响。细胞膜的自旋标记探针表明，在高浓度Na⁺或小球形疏水分子金刚烷酮中生长会导致细胞膜“流动性”增加，而在高浓度Ca²⁺中生长会使细胞膜的刚性略有增加。此外，细胞膜的磷脂组成取决于生长培养基中的NaCl浓度。然而，这些膜的变化均不会阻止感染性phi 6病毒颗粒的产生。
