Quatacker J
Cell Tissue Res. 1975 Aug 27;161(4):541-53. doi: 10.1007/BF00224143.
Horseradish peroxidase (HRP) was injected directly into the corpora lutea of rabbits on different days of pseudopregnancy. Young luteal cells have also been incubated "in vitro" in a medium containing horseradish peroxidase. In the "in vivo" experiments the 5 to 9 days old luteal cells take up far more horseradish peroxidase than the older ones (14-20 days). Different types of endocytic vacuoles, MvB's and also some DB's are already peroxidase positive shortly (15-20 min) after injection of the tracer. In the "in vitro" experiments the cells are more heavily loaded. The normal morphology of pale, dense and terminal MvB's is also described and staining with PTA at low pH provides further information on changes occurring in the MvB's. The various findings on multivesicular bodies are compared and two possible pathways for endocytic vacuoles are proposed: one to DB's the other to MvB's. The related phenomena of the uptake of material and the internalization of plasma membrane are discussed in the light of the possible function of endocytosis in luteal cells.
在假孕不同天数时，将辣根过氧化物酶（HRP）直接注射到兔的黄体中。年轻的黄体细胞也在含有辣根过氧化物酶的培养基中进行了“体外”培养。在“体内”实验中，5至9天龄的黄体细胞比年长的黄体细胞（14 - 20天）摄取的辣根过氧化物酶要多得多。注射示踪剂后不久（15 - 20分钟），不同类型的内吞小泡、多泡体（MvB）以及一些致密小体（DB）就已呈过氧化物酶阳性。在“体外”实验中，细胞负载更重。还描述了苍白、致密和终末多泡体的正常形态，并且在低pH值下用磷钨酸（PTA）染色可提供有关多泡体中发生变化的更多信息。对多泡体的各种发现进行了比较，并提出了内吞小泡的两种可能途径：一种通向致密小体，另一种通向多泡体。根据黄体细胞内吞作用的可能功能，讨论了物质摄取和质膜内化的相关现象。
