溶酶体在冠状病毒形态发生中的意义。
Significance of lysosomes in the morphogenesis of coronaviruses.
作者信息
Ducatelle R, Hoorens J
出版信息
Arch Virol. 1984;79(1-2):1-12. doi: 10.1007/BF01314299.
Abstract
Virus-containing electron-dense membrane-bound cytoplasmic bodies are described in tracheal epithelial cells of chickens infected with Infectious Bronchitis Virus and in intestinal epithelial cells of swine infected with Porcine Epidemic Diarrhea Virus. Using silver-methenamine staining, phosphotungstic acid staining and acid phosphatase enzyme cytochemical staining of ultra-thin sections, these bodies were shown to be virus-containing secondary lysosomes and residual bodies. The accumulation of viral particles in the lysosomes is suggested to possibly represent an intracellular defense mechanism. However, no morphological alterations were found indicating a destruction of the viruses by the lytic lysosomal enzymes.
摘要
在感染传染性支气管炎病毒的鸡的气管上皮细胞以及感染猪流行性腹泻病毒的猪的肠道上皮细胞中，发现了含有病毒的电子致密膜结合细胞质小体。通过对超薄切片进行六胺银染色、磷钨酸染色和酸性磷酸酶细胞化学染色，这些小体显示为含有病毒的次级溶酶体和残余小体。病毒颗粒在溶酶体中的积累可能代表一种细胞内防御机制。然而，未发现表明病毒被溶酶体裂解酶破坏的形态学改变。
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