Juravinski Cancer Centre, Physics Research, 699 Concession St., Hamilton, Ontario, L8V 5C2, Canada.
Phys Med Biol. 2013 Oct 21;58(20):7039-59. doi: 10.1088/0031-9155/58/20/7039. Epub 2013 Sep 20.
A novel technique is described that uses either time-resolved or steady state prompt photosensitizer fluorescence to measure local oxygen concentration. Solution experiments conducted with Al(III) phthalocyanine chloride tetrasulfonic acid confirmed that the steady state fluorescence signal is dependent on the oxygen concentration and fluence rate. A relationship between prompt sensitizer fluorescence and sensitizer triplet quenching efficiency is derived which does not require knowledge of the Stern-Volmer constant. Similar relationships are also derived for sensitizer delayed fluorescence and phosphorescence. An explicit photodynamic therapy (PDT) dose metric that incorporates light dosimetry, sensitizer dosimetry, and triplet quenching efficiency is introduced. All components of this metric can be determined by optical measurements.
介绍了一种新的技术，它使用时间分辨或稳态提示荧光剂荧光来测量局部氧浓度。用 Al(III)酞菁四磺酸氯进行的溶液实验证实，稳态荧光信号取决于氧浓度和光强度。推导出了与瞬态荧光剂荧光和荧光剂三重态猝灭效率的关系，该关系不需要知道 Stern-Volmer 常数。类似的关系也适用于荧光剂延迟荧光和磷光。引入了一种包含光剂量学、荧光剂剂量学和三重态猝灭效率的明确光动力疗法（PDT）剂量指标。该指标的所有成分都可以通过光学测量来确定。
