Cochrane A W, Chen C H, Rosen C A
Department of Molecular Oncology & Virology, Roche Institute of Molecular Biology, Nutley, NJ 07110.
Proc Natl Acad Sci U S A. 1990 Feb;87(3):1198-202. doi: 10.1073/pnas.87.3.1198.
A region of potential complex secondary structure within the human immunodeficiency virus env mRNA has been implicated in Rev-mediated export of viral structural mRNAs from the nucleus to the cytoplasm. By using an RNase protection gel-mobility-shift assay, we demonstrate that purified Rev protein forms a stable complex with this Rev-responsive RNA. RNAs with mutations designed to disrupt formation of a predicted stem structure no longer interact with Rev. However, Rev binding is restored upon annealing of the two complementary RNAs that make up the stem. These results suggest that direct interaction of Rev with the Rev-responsive element could facilitate transport of human immunodeficiency virus structural mRNAs from the nucleus to the cytoplasm.
人类免疫缺陷病毒env mRNA内潜在的复杂二级结构区域与Rev介导的病毒结构mRNA从细胞核输出到细胞质有关。通过使用核糖核酸酶保护凝胶迁移率变动分析，我们证明纯化的Rev蛋白与这种Rev反应性RNA形成稳定的复合物。设计用于破坏预测茎结构形成的突变RNA不再与Rev相互作用。然而，当构成茎的两个互补RNA退火时，Rev结合得以恢复。这些结果表明，Rev与Rev反应元件的直接相互作用可能促进人类免疫缺陷病毒结构mRNA从细胞核到细胞质的运输。
