Dayton E T, Powell D M, Dayton A I
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Disease, Bethesda, MD 20892.
Science. 1989 Dec 22;246(4937):1625-9. doi: 10.1126/science.2688093.
Expression of high levels of the structural proteins of the human immunodeficiency virus type 1 (HIV-1) requires the presence of the protein encoded by the rev open reading frame (Rev) and its associated target sequence CAR (cis anti-repression sequence) which is present in the env region of viral RNA. Extensive mutagenesis demonstrated that CAR has a complex secondary structure consisting of a central stem and five stem/loops. Disruption of any of these structures severely impaired the Rev response, but many of the stem/loops contain material that was unnecessary for Rev regulation and must be retained in these structures to avoid disturbing adjacent structures critical for CAR function. Probably no more than two of the described structural components are involved in sequence-specific recognition by regulatory proteins.
1型人类免疫缺陷病毒（HIV-1）结构蛋白的高水平表达需要存在由rev开放阅读框（Rev）编码的蛋白质及其相关的靶序列CAR（顺式抗阻抑序列），该序列存在于病毒RNA的env区域。广泛的诱变表明，CAR具有复杂的二级结构，由一个中央茎和五个茎环组成。破坏这些结构中的任何一个都会严重损害Rev反应，但许多茎环包含对Rev调节不必要的物质，必须保留在这些结构中以避免干扰对CAR功能至关重要的相邻结构。可能不超过两个所述结构成分参与调节蛋白的序列特异性识别。
