Department of Medical Biochemistry, Medical University of Lodz, Mazowiecka 6/8, 92-215 Lodz, Poland.
Virus Res. 2013 Dec 26;178(2):539-42. doi: 10.1016/j.virusres.2013.09.019. Epub 2013 Sep 18.
Peripheral blood mononuclear cells (PBMCs) constitute the main extrahepatic place of, hepatitis C virus (HCV) replication. We aimed to determine the impact of CHC infection and microRNA-, 122 expression on cholesterol expression in PBMCs. HCV RNA strand, intracellular cholesterol, HMGCoA, reductase and miR-122 expression in PBMC were determined in 54 CHC patients. The study shows that significant decrease of intracellular cholesterol level in PBMC (p=0.000000), accompanied by serum hypocholesterolemia is the characteristic feature of chronic hepatitis C infection. Although, microRNA-122 expression was detectable in PBMCs of CHC patients (52.5%), the alteration of intracellular cholesterol level was independent of miR-122 expression.
外周血单个核细胞（PBMC）是丙型肝炎病毒（HCV）复制的主要肝外场所。我们旨在确定 CHC 感染和 microRNA-122 表达对 PBMC 中胆固醇表达的影响。在 54 例 CHC 患者中测定了 HCV RNA 链、PBMC 中的细胞内胆固醇、HMGCoA 还原酶和 miR-122 的表达。研究表明，慢性丙型肝炎感染的特征是 PBMC 中细胞内胆固醇水平显著降低（p=0.000000），同时伴有血清胆固醇降低。尽管在 CHC 患者的 PBMC 中可检测到 microRNA-122 的表达（52.5%），但细胞内胆固醇水平的改变与 miR-122 的表达无关。
