Duke University, Department of Biomedical Engineering, Durham, North Carolina 27708bUniversity of Auckland, Auckland Bioengineering Institute, 70 Symonds Street, Auckland 1142, New Zealand.
J Biomed Opt. 2013 Sep;18(9):096013. doi: 10.1117/1.JBO.18.9.096013.
Atherosclerosis is a leading cause of mortality in industrialized countries. In addition to "traditional" systemic risk factors for atherosclerosis, the geometry and motion of coronary arteries may contribute to individual susceptibility to the development and progression of disease in these vessels. To be able to test this, we have developed a high-speed (∼40 frames per second) microscope-based stereo-imaging system to quantify the motion of epicardial coronary arteries of mice. Using near-infrared nontargeted quantum dots as an imaging contrast agent, we synchronously acquired paired images of a surgically exposed murine heart, from which the three-dimensional geometry of the coronary arteries was reconstructed. The reconstructed geometry was tracked frame by frame through the cardiac cycle to quantify the in vivo motion of the vessel, from which displacements, curvature, and torsion parameters were derived. Illustrative results for a C57BL/6J mouse are presented.
动脉粥样硬化是工业化国家死亡的主要原因。除了动脉粥样硬化的“传统”系统性危险因素外，冠状动脉的几何形状和运动也可能导致这些血管中疾病的发展和进展的个体易感性。为了能够对此进行测试，我们开发了一种高速（约每秒 40 帧）基于显微镜的立体成像系统，以定量测量小鼠心外膜冠状动脉的运动。我们使用近红外非靶向量子点作为成像对比剂，从手术暴露的鼠心同步获取成对的图像，从中重建冠状动脉的三维几何形状。通过心脏周期逐帧跟踪重建的几何形状，以定量测量血管的体内运动，从中得出位移、曲率和扭转参数。展示了一只 C57BL/6J 小鼠的说明性结果。
