Department of Mechanical and Industrial Engineering, Ryerson University, Toronto, ON M5B 2K3, Canada.
J Biomed Nanotechnol. 2013 Nov;9(11):1837-46. doi: 10.1166/jbn.2013.1679.
The current challenge in tissue engineering is to design a platform that can provide appropriate topography and suitable surface chemistry to encourage desired cellular activities and to guide 3-D tissue regeneration. Compared with traditional cell culture materials, 3-D nanofibrous platforms offer a superior environment for promoting cell functions by mimicking the architecture of extracellular matrix (ECM). In this study, we present a technique to engineer freestanding 3-D titania nanofibrous structures on titanium substrates using femtosecond laser processing. The crystallinity, surface adhesion, and surface energy of the synthesized nanostructures are discussed. The effects of synthesized nanoarchitectures on the proliferation, morphology, and viability of MC3T3-E1 mouse osteoblast-like cells and NIH 3T3 mouse embryonic fibroblasts are investigated. The nanofibrous structures show high surface energy and hydrophilicity. The results from in vitro studies reveal that the titania nanofibrous architectures possess excellent biocompatibility and significantly enhances proliferation of both cell lines compared to untreated titanium specimens. Study of the cell morphology shows dynamic cell migration and attachment on the titania nanofibrous architecture. The bioactivity and biocompatibility of the engineered 3-D nanostructures suggest noticeable perspective for developing bio-functionalized scaffolds and implantable materials in regenerative medicine and clinical tissue engineering.
当前组织工程学面临的挑战是设计一个能够提供适当形貌和合适表面化学性质的平台，以促进所需的细胞活动并指导 3D 组织再生。与传统的细胞培养材料相比，3D 纳米纤维平台通过模拟细胞外基质（ECM）的结构，为促进细胞功能提供了优越的环境。在本研究中，我们提出了一种使用飞秒激光加工在钛基体上构建独立式 3D 二氧化钛纳米纤维结构的技术。讨论了所合成的纳米结构的结晶度、表面附着力和表面能。研究了合成的纳米结构对 MC3T3-E1 小鼠成骨样细胞和 NIH 3T3 小鼠胚胎成纤维细胞增殖、形态和活力的影响。纳米纤维结构具有高表面能和高亲水性。体外研究结果表明，与未经处理的钛样品相比，二氧化钛纳米纤维结构具有优异的生物相容性，并显著促进了两种细胞系的增殖。细胞形态研究表明，细胞在二氧化钛纳米纤维结构上表现出动态的迁移和附着。工程 3D 纳米结构的生物活性和生物相容性表明，其在再生医学和临床组织工程中开发生物功能化支架和可植入材料方面具有显著的应用前景。
