Department of Biomaterials, Max Planck Institute of Colloids and Interfaces, 14476, Potsdam-Golm, Germany.
Institute for Chemistry and Biochemistry, Freie Universität Berlin, 14195, Berlin, Germany.
Adv Healthc Mater. 2017 Aug;6(15). doi: 10.1002/adhm.201601244. Epub 2017 Mar 30.
Surface structuring of titanium-based implants is known to modulate the behavior of adherent cells, but the influence of different nanotopographies is poorly understood. The aim is to investigate preosteoblast proliferation, adhesion, morphology, and migration on surfaces with similar surface chemistry but distinct nanotopographical features. Sonochemical treatment and anodic oxidation are employed to fabricate disordered, mesoporous titania (TMS) and ordered titania nanotubular (TNT) topographies on titanium, respectively. Morphological evaluation reveals that cells are polygonal and well-spread on TMS, but display an elongated, fibroblast-like morphology on TNT surfaces, while they are much flatter on glass. Both nanostructured surfaces impair cell adhesion, but TMS is more favorable for cell growth due to its support of cell attachment and spreading in contrast to TNT. A quantitative wound healing assay in combination with live-cell imaging reveals that cell migration on TMS surfaces has a more collective character than on other surfaces, probably due to a closer proximity between neighboring migrating cells on TMS. The results indicate distinctly different cell adhesion and migration on ordered and disordered titania nanotopographies, providing important information that can be used in optimizing titanium-based scaffold design to foster bone tissue growth and repair while allowing for the encapsulation of drugs into porous titania layer.
钛基植入物的表面结构已被证明可以调节附着细胞的行为，但不同纳米形貌的影响还不太清楚。本研究旨在探讨具有相似表面化学性质但具有不同纳米形貌特征的表面上，前成骨细胞的增殖、黏附、形态和迁移。采用超声化学处理和阳极氧化法分别在钛上制备无序介孔二氧化钛（TMS）和有序二氧化钛纳米管（TNT）形貌。形态学评价表明，细胞在 TMS 上呈多边形且铺展良好，但在 TNT 表面呈长梭形，类似于成纤维细胞，而在玻璃上则更扁平。两种纳米结构表面都能抑制细胞黏附，但 TMS 更有利于细胞生长，因为它支持细胞附着和铺展，而 TNT 则不支持。结合活细胞成像的定量划痕愈合实验表明，TMS 表面上的细胞迁移具有更强的集体特征，这可能是由于 TMS 表面上相邻迁移细胞之间的距离更近。这些结果表明，在有序和无序的二氧化钛纳米形貌上，细胞黏附和迁移具有明显不同的特征，为优化基于钛的支架设计提供了重要信息，以促进骨组织生长和修复，同时允许将药物封装到多孔二氧化钛层中。
