College of Engineering, Swansea University, Singleton Park, Swansea, UK; University Kuala Lumpur, British Malaysian Institute, Gombak, Selangor, Malaysia.
Biosens Bioelectron. 2014 Feb 15;52:216-24. doi: 10.1016/j.bios.2013.08.030. Epub 2013 Sep 3.
In this article we present ultra-sensitive, silicon nanowire (SiNW)-based biosensor devices for the detection of disease biomarkers. An electrochemically induced functionalisation method has been employed to graft antibodies targeted against the prostate cancer risk biomarker 8-hydroxydeoxyguanosine (8-OHdG) to SiNW surfaces. The antibody-functionalised SiNW sensor has been used to detect binding of the 8-OHdG biomarker to the SiNW surface within seconds of exposure. Detection of 8-OHdG concentrations as low as 1 ng/ml (3.5 nM) has been demonstrated. The active device has been bonded to a disposable printed circuit which can be inserted into an electronic readout system as part of an integrated Point of Care (POC) diagnostic. The speed, sensitivity and ease of detection of biomarkers using SiNW sensors render them ideal for eventual POC diagnostics.
在本文中，我们展示了基于硅纳米线 (SiNW) 的超灵敏生物传感器设备，用于检测疾病生物标志物。我们采用电化学诱导的功能化方法将针对前列腺癌风险生物标志物 8-羟基脱氧鸟苷 (8-OHdG) 的抗体嫁接到 SiNW 表面。该抗体功能化的 SiNW 传感器已被用于在暴露后几秒钟内检测 8-OHdG 生物标志物与 SiNW 表面的结合。已经证明可以检测到低至 1ng/ml（3.5nM）的 8-OHdG 浓度。该有源器件已与一次性印刷电路板结合，可以插入电子读出系统中，作为集成即时诊断 (POC) 诊断的一部分。SiNW 传感器在生物标志物检测方面的速度、灵敏度和易用性使其成为最终 POC 诊断的理想选择。
