用适体和抗体敏化的纳米带生物传感器检测丙型肝炎病毒核心抗原的性能研究

The Study of Performance of a Nanoribbon Biosensor, Sensitized with Aptamers and Antibodies, upon Detection of Core Antigen of Hepatitis C Virus.

作者信息

Ivanov Yuri D, Malsagova Kristina A, Goldaeva Kristina V, Pleshakova Tatyana O, Kozlov Andrey F, Galiullin Rafael A, Shumov Ivan D, Popov Vladimir P, Abramova Irina K, Ziborov Vadim S, Petrov Oleg F, Dolgoborodov Alexander Yu, Archakov Alexander I

机构信息

Institute of Biomedical Chemistry (IBMC), 119121 Moscow, Russia.

Joint Institute for High Temperatures of Russian Academy of Sciences, 125412 Moscow, Russia.

出版信息

Micromachines (Basel). 2023 Oct 19;14(10):1946. doi: 10.3390/mi14101946.

DOI:10.3390/mi14101946

PMID:37893383

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10609547/

Abstract

The development of highly sensitive diagnostic systems for the early revelation of diseases in humans is one of the most important tasks of modern biomedical research, and the detection of the core antigen of the hepatitis C virus (HCVcoreAg)-a protein marker of the hepatitis C virus-is just the case. Our study is aimed at testing the performance of the nanoribbon biosensor in the case of the use of two different types of molecular probes: the antibodies and the aptamers against HCVcoreAg. The nanoribbon sensor chips employed are based on "silicon-on-insulator structures" (SOI-NR). Two different HCVcoreAg preparations are tested: recombinant β-galactosidase-conjugated HCVcoreAg ("Virogen", Watertown, MA, USA) and recombinant HCVcoreAg ("Vector-Best", Novosibirsk, Russia). Upon the detection of either type of antigen preparation, the lowest concentration of the antigen detectable in buffer with pH 5.1 was found to be approximately equal, amounting to ~10 M. This value was similar upon the use of either type of molecular probes.

摘要

开发用于早期发现人类疾病的高灵敏度诊断系统是现代生物医学研究的最重要任务之一，而丙型肝炎病毒核心抗原（HCVcoreAg）——丙型肝炎病毒的一种蛋白质标志物——的检测正是这样一个实例。我们的研究旨在测试纳米带生物传感器在使用两种不同类型分子探针（即针对HCVcoreAg的抗体和适体）时的性能。所采用的纳米带传感器芯片基于“绝缘体上硅结构”（SOI-NR）。测试了两种不同的HCVcoreAg制剂：重组β-半乳糖苷酶偶联的HCVcoreAg（“Virogen”，美国马萨诸塞州沃特敦）和重组HCVcoreAg（“Vector-Best”，俄罗斯新西伯利亚）。在检测任何一种抗原制剂时，发现在pH 5.1的缓冲液中可检测到的最低抗原浓度大致相等，约为~10 M。使用任何一种分子探针时，该值都相似。