Zhou Gui-Xiong, Chen Guan-Yi, Yan Bei-Bei
State Key Laboratory of Engines, School of Environmental Science and Technology, Tianjin University, Tianjin, 300072, China,
Biotechnol Lett. 2014 Jan;36(1):63-8. doi: 10.1007/s10529-013-1336-x. Epub 2013 Sep 24.
Biodiesel production by immobilized Rhizopus oryzae lipase in magnetic chitosan microspheres (MCMs) was carried out using soybean oil and methanol in a magnetically-stabilized, fluidized bed reactor (MSFBR). The maximum content of methyl ester in the reaction mixture reached 91.3 (w/v) at a fluid flow rate of 25 ml/min and a magnetic field intensity of 150 Oe. In addition, the MCMs-immobilized lipase in the reactor showed excellent reusability, retaining 82 % productivity even after six batches, which was much better than that in a conventional fluidized bed reactor. These results suggested that a MSFRB using MCMs-immobilized lipase is a promising method for biodiesel production.
在磁稳定流化床反应器（MSFBR）中，使用大豆油和甲醇，通过固定在磁性壳聚糖微球（MCMs）中的米根霉脂肪酶进行生物柴油生产。在流体流速为25毫升/分钟和磁场强度为150奥斯特的条件下，反应混合物中甲酯的最大含量达到91.3（w/v）。此外，反应器中固定在MCMs上的脂肪酶表现出优异的可重复使用性，即使在六批反应后仍保留82%的生产率，这比传统流化床反应器中的情况要好得多。这些结果表明，使用固定在MCMs上的脂肪酶的MSFRB是一种很有前途的生物柴油生产方法。
