Chen Hsiao-Ching, Ju Hen-Yi, Wu Tsung-Ta, Liu Yung-Chuan, Lee Chih-Chen, Chang Cheng, Chung Yi-Lin, Shieh Chwen-Jen
Department and Graduate Program of Bioindustry Technology, Dayeh University, Chang-Hua, Taiwan.
J Biomed Biotechnol. 2011;2011. doi: 10.1155/2011/950725. Epub 2010 Sep 28.
An optimal continuous production of biodiesel by methanolysis of soybean oil in a packed-bed reactor was developed using immobilized lipase (Novozym 435) as a catalyst in a tert-butanol solvent system. Response surface methodology (RSM) and Box-Behnken design were employed to evaluate the effects of reaction temperature, flow rate, and substrate molar ratio on the molar conversion of biodiesel. The results showed that flow rate and temperature have significant effects on the percentage of molar conversion. On the basis of ridge max analysis, the optimum conditions were as follows: flow rate 0.1 mL/min, temperature 52.1°C, and substrate molar ratio 1 : 4. The predicted and experimental values of molar conversion were 83.31 ± 2.07% and 82.81 ± .98%, respectively. Furthermore, the continuous process over 30 days showed no appreciable decrease in the molar conversion. The paper demonstrates the applicability of using immobilized lipase and a packed-bed reactor for continuous biodiesel synthesis.
以固定化脂肪酶（诺维信435）为催化剂，在叔丁醇溶剂体系中，利用填充床反应器通过大豆油甲醇解实现了生物柴油的优化连续生产。采用响应面法（RSM）和Box-Behnken设计来评估反应温度、流速和底物摩尔比对生物柴油摩尔转化率的影响。结果表明，流速和温度对摩尔转化率百分比有显著影响。基于岭脊最大值分析，最佳条件如下：流速0.1毫升/分钟，温度52.1℃，底物摩尔比1∶4。摩尔转化率的预测值和实验值分别为83.31±2.07%和82.81±0.98%。此外，超过30天的连续过程显示摩尔转化率没有明显下降。本文证明了使用固定化脂肪酶和填充床反应器进行连续生物柴油合成的适用性。
