Medical Department of Neurology, Affiliated Hospital of Beihua University, Jilin, 132011, People's Republic of China,
J Neurooncol. 2013 Dec;115(3):375-80. doi: 10.1007/s11060-013-1243-y. Epub 2013 Sep 24.
MicroRNAs are known as non-coding RNAs that regulate the expression of target mRNA. Accumulating evidence has indicated that microRNA expression in human malignancies can be utilized as a prognostic marker for patients. However, the prognostic value of miR-650 in human glioma has not been investigated yet. In the present investigation, we have recruited 168 cases glioma specimens and 21 normal control brain specimens. Quantitative real-time PCR was carried out to investigate the expression of miR-650. Kaplan-Meier analysis and Cox's proportional hazards model was used to evaluate the association of miR-650 with prognosis of glioma patients. Results showed that miR-650 expression was increased in glioma compared with normal control specimens (P < 0.001). It was also found that miR-650 expression was related to World Health Organization grade and Karnofsky performance score (KPS) for high expression was more frequently detected in glioma of high grade or low KPS score (P < 0.001). The prognosis of glioma with high miR-650 expression was significantly worse compared with that of glioma with low miR-650 expression. These results proved that miR-650 expression was a significant prognostic indicator in glioma, which may suggest new management of human glioma.
微 RNA 被称为非编码 RNA，可以调节靶 mRNA 的表达。越来越多的证据表明，人类恶性肿瘤中的微 RNA 表达可以作为患者的预后标志物。然而，miR-650 在人类脑胶质瘤中的预后价值尚未得到研究。在本研究中，我们招募了 168 例脑胶质瘤标本和 21 例正常对照脑标本。采用实时定量 PCR 检测 miR-650 的表达。采用 Kaplan-Meier 分析和 Cox 比例风险模型评估 miR-650 与脑胶质瘤患者预后的关系。结果表明，miR-650 在脑胶质瘤中的表达高于正常对照标本（P < 0.001）。还发现 miR-650 的表达与世界卫生组织（WHO）分级和 Karnofsky 表现评分（KPS）有关，高表达更频繁地出现在高级别或低 KPS 评分的脑胶质瘤中（P < 0.001）。高 miR-650 表达的脑胶质瘤患者的预后明显差于低 miR-650 表达的脑胶质瘤患者。这些结果证明 miR-650 的表达是脑胶质瘤的一个显著预后指标，可能为人类脑胶质瘤的治疗提供新的思路。
