Dahlén G, Renvert S, Wikström M, Egelberg J
Department of Oral Microbiology, Faculty of Odontology, University of Göteborg, Sweden.
J Clin Periodontol. 1990 Feb;17(2):73-7. doi: 10.1111/j.1600-051x.1990.tb01065.x.
Duplicate microbiological samples, were taken 1 week apart using the paper point technique from a total of 112 untreated periodontal pockets greater than 6 mm deep in 16 adult periodontal patients. Duplicate samples were also obtained from these sites 6 months following a therapy of oral hygiene instruction and supra- and subgingival debridement. The reproducibility of the total viable counts and the reproducibility of the proportions of various groups or species of microorganisms were studied from these duplicate samples. The results demonstrated an acceptable degree of reproducibility for the recovery of Actinobacillus actinomycetemcomitans and Bacteroides gingivalis. For the total viable counts and for the other investigated bacterial groups, including Bacteroides intermedius, unacceptable levels of reproducibility were observed.
采用纸尖技术，在16名成年牙周病患者的总共112个深度大于6mm的未经治疗的牙周袋中，每隔1周采集重复的微生物样本。在进行口腔卫生指导以及龈上和龈下清创治疗6个月后，也从这些部位采集重复样本。从这些重复样本中研究了总活菌数的可重复性以及各种微生物群或菌种比例的可重复性。结果表明，伴放线放线杆菌和牙龈拟杆菌的回收率具有可接受的可重复性程度。对于总活菌数以及包括中间普氏菌在内的其他研究细菌组，观察到了不可接受的可重复性水平。
