Department of Immunotechnology, Lund University , Medicon Village, Lund, Sweden.
J Proteome Res. 2013 Dec 6;12(12):5943-53. doi: 10.1021/pr4003245. Epub 2013 Oct 9.
Proteomics, the large-scale analysis of proteins, is a rapidly evolving field with an increasing number of key clinical applications, such as diagnosis, prognosis, and classification. In order to generate complete protein expression profiles, or protein atlases, any crude sample format must be addressable in a rapid, multiplex, and sensitive manner. A common and clinically central sample format, formalin-fixed, paraffin-embedded (FFPE) tissue material, holds great potential as a source for disease-associated biomarker signatures. However, despite major efforts, extraction and subsequent profiling of proteins from FFPE tissue has proven to be challenging. In this proof-of-concept study, we have demonstrated for the first time that proteins could be extracted, labeled, and subsequently profiled in a multiplex, sensitive, and reproducible manner using recombinant scFv antibody microarrays. Thus, we have added FFPE samples to the list of sample formats available for high-throughput analysis by affinity proteomics, paving the way for the next generation of biomarker-driven discovery projects.
蛋白质组学是蛋白质的大规模分析，是一个快速发展的领域，其具有越来越多的关键临床应用，例如诊断、预后和分类。为了生成完整的蛋白质表达谱或蛋白质图谱，任何粗制的样本形式都必须以快速、多重和敏感的方式进行处理。福尔马林固定、石蜡包埋（FFPE）组织材料是一种常见且在临床上具有中心地位的样本形式，作为疾病相关生物标志物特征的来源具有巨大的潜力。然而，尽管付出了巨大努力，但从 FFPE 组织中提取和随后进行蛋白质分析已被证明是具有挑战性的。在这项概念验证研究中，我们首次证明可以使用重组 scFv 抗体微阵列以多重、敏感和可重复的方式提取、标记和随后分析蛋白质。因此，我们已经将 FFPE 样本添加到可通过亲和蛋白质组学进行高通量分析的样本格式列表中，为下一代基于生物标志物的发现项目铺平了道路。
