Pereira U P, Soares S C, Blom J, Leal C A G, Ramos R T J, Guimarães L C, Oliveira L C, Almeida S S, Hassan S S, Santos A R, Miyoshi A, Silva A, Tauch A, Barh D, Azevedo V, Figueiredo H C P
AQUAVET - Laboratório de Doenças de Animais Aquáticos, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Genet Mol Res. 2013 Aug 12;12(3):2902-12. doi: 10.4238/2013.August.12.6.
Streptococcus agalactiae (Lancefield group B; group B streptococci) is a major pathogen that causes meningoencephalitis in fish, mastitis in cows, and neonatal sepsis and meningitis in humans. The available prophylactic measures for conserving human and animal health are not totally effective and have limitations. Effective vaccines against the different serotypes or genotypes of pathogenic strains from the various hosts would be useful. We used an in silico strategy to identify conserved vaccine candidates in 15 genomes of group B streptococci strains isolated from human, bovine, and fish samples. The degree of conservation, subcellular localization, and immunogenic potential of S. agalactiae proteins were investigated. We identified 36 antigenic proteins that were conserved in all 15 genomes. Among these proteins, 5 and 23 were shared only by human or fish strains, respectively. These potential vaccine targets may help develop effective vaccines that will help prevent S. agalactiae infection.
无乳链球菌（兰斯菲尔德B组；B组链球菌）是一种主要病原体，可导致鱼类脑膜脑炎、奶牛乳腺炎以及人类新生儿败血症和脑膜炎。现有的保护人类和动物健康的预防措施并不完全有效且存在局限性。针对来自不同宿主的致病菌株的不同血清型或基因型的有效疫苗将会很有用。我们采用计算机模拟策略，在从人类、牛和鱼类样本中分离出的15个B组链球菌菌株基因组中鉴定保守的疫苗候选物。研究了无乳链球菌蛋白质的保守程度、亚细胞定位和免疫原性潜力。我们鉴定出在所有15个基因组中均保守的36种抗原蛋白。在这些蛋白质中，分别有5种和23种仅在人类或鱼类菌株中共有。这些潜在的疫苗靶点可能有助于开发有效的疫苗，从而有助于预防无乳链球菌感染。
