Islam Sk Injamamul, Mou Moslema Jahan, Sanjida Saloa
Department of Fisheries and Marine Bioscience, Faculty of Biological Science, Jashore University of Science and Technology, Jashore, 7408, Bangladesh.
Center of Excellence in Fish Infectious Diseases (CE FID), Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.
J Genet Eng Biotechnol. 2022 Aug 8;20(1):118. doi: 10.1186/s43141-022-00391-8.
Aeromonas veronii is one of the most common pathogens of freshwater fishes that cause sepsis and ulcers. There are increasing numbers of cases showing that it is a significant zoonotic and aquatic agent. Epidemiological studies have shown that A. veronii virulence and drug tolerance have both increased over the last few years as a result of epidemiological investigations. Cadaverine reverse transporter (CadB) and maltoporin (LamB protein) contribute to the virulence of A. veronii TH0426. TH0426 strain is currently showing severe cases on fish species, and its resistance against therapeutic has been increasing. Despite these devastating complications, there is still no effective cure or vaccine for this strain of A.veronii.
In this regard, an immunoinformatic method was used to generate an epitope-based vaccine against this pathogen. The immunodominant epitopes were identified using the CadB and LamB protein of A. veronii. The final constructed vaccine sequence was developed to be immunogenic, non-allergenic as well as have better solubility. Molecular dynamic simulation revealed significant binding stability and structural compactness. Finally, using Escherichia coli K12 as a model, codon optimization yielded ideal GC content and a higher CAI value, which was then included in the cloning vector pET2+ (a).
Altogether, our outcomes imply that the proposed peptide vaccine might be a good option for A. veronii TH0426 prophylaxis.
维氏气单胞菌是淡水鱼中最常见的病原体之一，可导致败血症和溃疡。越来越多的病例表明，它是一种重要的人畜共患病原体和水生病原体。流行病学研究表明，在过去几年的流行病学调查中，维氏气单胞菌的毒力和耐药性均有所增加。尸胺逆向转运蛋白（CadB）和麦芽寡糖孔蛋白（LamB蛋白）有助于维氏气单胞菌TH0426的毒力。TH0426菌株目前在鱼类中表现出严重病例，并且其对治疗的耐药性一直在增加。尽管存在这些毁灭性的并发症，但对于这种维氏气单胞菌菌株仍然没有有效的治疗方法或疫苗。
在这方面，采用免疫信息学方法针对这种病原体开发了一种基于表位的疫苗。使用维氏气单胞菌的CadB和LamB蛋白鉴定了免疫显性表位。最终构建的疫苗序列具有免疫原性、无致敏性且溶解性更好。分子动力学模拟显示出显著的结合稳定性和结构紧凑性。最后，以大肠杆菌K12为模型，密码子优化产生了理想的GC含量和更高的CAI值，然后将其包含在克隆载体pET2 +（a）中。
总之，我们的结果表明，所提出的肽疫苗可能是预防维氏气单胞菌TH0426的一个不错选择。
