Laboratory for Autoimmune Regulation, Research Center for Allergy and Immunology, RIKEN, Yokohama City, Kanagawa, Japan ; Laboratory for Cell Function and Dynamics, Brain Science Institute, RIKEN, Wako City, Saitama, Japan ; Center for Innovation in Immunoregulative Technology and Therapeutics, Kyoto University Graduate School of Medicine, Kyoto City, Japan.
PLoS One. 2013 Sep 16;8(9):e73801. doi: 10.1371/journal.pone.0073801. eCollection 2013.
A transgenic mouse line expressing Fucci (fluorescent ubiquitination-based cell-cycle indicator) probes allows us to monitor the cell cycle in the hematopoietic system. Two populations with high and low intensities of Fucci signals for Cdt1(30/120) accumulation were identified by FACS analysis, and these correspond to quiescent G0 and cycling G1 cells, respectively. We observed the transition of immune cells between quiescent and proliferative phases in lymphoid organs during differentiation and immune responses.
一种表达 Fucci（荧光泛素细胞周期指示剂）探针的转基因小鼠品系使我们能够监测造血系统中的细胞周期。通过 FACS 分析鉴定出 Cdt1(30/120)积累的 Fucci 信号强度高和低的两个群体，它们分别对应于静止的 G0 和循环的 G1 细胞。我们观察到在分化和免疫反应过程中，淋巴器官中的免疫细胞在静止和增殖期之间的转变。
