Department of Pharmacology, School of Basic Medicine, Hebei Medical University, Shijiazhuang, China.
PLoS One. 2013 Sep 16;8(9):e74318. doi: 10.1371/journal.pone.0074318. eCollection 2013.
Excessive iron can accumulate in the kidney and induce tissue damage. Danshen (Salvia miltiorrhiza) injection is a traditional Chinese medicinal preparation used for preventing and treating chronic renal failure. The aim of the present study was to evaluate the effects of treatment with Danshen injection on iron overload-induced kidney damage.
Mice were mock-treated with saline (control group) or given a single dose of iron dextran without treatment (iron overload group, 50 mg/kg/day for 2 weeks) or with daily treatments of low-dose Danshen (3 g/kg/day), high-dose Danshen (6 g/kg/day) or deferoxamine (100 mg/kg/day).
Treatment of iron-overloaded mice with Danshen injection led to significant improvements of body weight and decreased iron levels in the kidney. Danshen injection treatment also reduced concentrations of blood urea nitrogen, creatinine and malondialdehyde and enhanced glutathione peroxidase and superoxide dismutase activities. Histopathological examinations showed that Danshen injection ameliorated pathological changes and reduced iron deposition in kidneys of iron overloaded mice. Furthermore, the treatment was demonstrated to suppress apoptosis in nephrocytes.
These results indicated that Danshen injection exerted significant renal protective effects in iron-overloaded mice, which were closely associated with the decrease of iron deposition and suppression of lipid peroxidation and apoptosis in the kidney.
过量的铁会在肾脏中蓄积并导致组织损伤。丹参注射液是一种用于预防和治疗慢性肾衰竭的传统中药制剂。本研究旨在评估丹参注射液治疗铁过载诱导的肾脏损伤的效果。
小鼠用生理盐水模拟处理（对照组）或给予铁葡聚糖单次剂量而未治疗（铁过载组，50mg/kg/天，持续 2 周）或每日给予低剂量丹参（3g/kg/天）、高剂量丹参（6g/kg/天）或去铁胺（100mg/kg/天）治疗。
丹参注射液治疗铁过载小鼠可显著改善体重，并降低肾脏中的铁含量。丹参注射液治疗还降低了血尿素氮、肌酐和丙二醛的浓度，并增强了谷胱甘肽过氧化物酶和超氧化物歧化酶的活性。组织病理学检查显示，丹参注射液可改善铁过载小鼠肾脏的病理变化并减少铁沉积。此外，该治疗方法还被证明可抑制肾细胞凋亡。
这些结果表明，丹参注射液对铁过载小鼠具有显著的肾脏保护作用，这与肾脏中铁沉积的减少以及脂质过氧化和细胞凋亡的抑制密切相关。
