Li Lei, Zhang Yuanyuan, Ma Juanjuan, Dong Weichong, Song Qiongtao, Zhang Jianping, Chu Li
Department of Pharmacology, School of Basic Medicine, Hebei Medical University, 326 Xinshi South Road, Shijiazhuang, Hebei 050091, China ; Department of Internal Medicine, Baoding First Hospital of Traditional Chinese Medicine, Yuhua Western Road, Baoding, Hebei 071000, China.
Department of Pharmacology, School of Basic Medicine, Hebei Medical University, 326 Xinshi South Road, Shijiazhuang, Hebei 050091, China.
ScientificWorldJournal. 2014 Feb 13;2014:572697. doi: 10.1155/2014/572697. eCollection 2014.
Exposure to lead (Pb) can induce kidney injury and our recent studies have found that Salvia miltiorrhiza (SM) injection, a traditional Chinese medicine, could protect against the organ injury induced by iron overload. This study was designed to investigate the protective effects of SM injection on nephrotoxicity induced by Pb acetate in mice and to elucidate the potential mechanism(s). Healthy male mice were randomly divided into four groups: control, Pb, low-dose Salvia miltiorrhiza (L-SM), and high-dose Salvia miltiorrhiza (H-SM). SM injection dose dependently reduced the Pb accumulation in the kidney, decreased kidney coefficients, and ameliorated renal structure and function from the morphology analysis. Meanwhile, SM administration downregulated serum levels of blood urea nitrogen (BUN) and creatinine (CR), decreased malondialdehyde (MAD) content, and increased activities of super oxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the kidney homogenate. Moreover, SM injection reduced the level of renal apoptosis by immunohistochemical staining analysis. Our findings implicate the therapeutic potential of SM injection for Pb-induced nephrotoxicity, which were at least partly due to the decrease of Pb accumulation, inhibition of lipid peroxidation, and suppression of renal apoptosis. These results provided preliminary experimental support for Danshen as a therapeutic drug for Pb poisoning diseases.
接触铅（Pb）可导致肾损伤，我们最近的研究发现，中药丹参注射液可预防铁过载诱导的器官损伤。本研究旨在探讨丹参注射液对醋酸铅诱导的小鼠肾毒性的保护作用，并阐明其潜在机制。健康雄性小鼠随机分为四组：对照组、铅组、低剂量丹参（L-SM）组和高剂量丹参（H-SM）组。从形态学分析来看，丹参注射液剂量依赖性地降低了肾脏中铅的蓄积，降低了肾脏系数，并改善了肾脏结构和功能。同时，丹参给药下调了血清尿素氮（BUN）和肌酐（CR）水平，降低了丙二醛（MAD）含量，并提高了肾脏匀浆中超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性。此外，通过免疫组化染色分析，丹参注射液降低了肾脏凋亡水平。我们的研究结果表明丹参注射液对铅诱导的肾毒性具有治疗潜力，这至少部分归因于铅蓄积的减少、脂质过氧化的抑制和肾脏凋亡的抑制。这些结果为丹参作为铅中毒疾病的治疗药物提供了初步的实验支持。
