Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University , Beijing, China .
Foodborne Pathog Dis. 2014 Jan;11(1):75-7. doi: 10.1089/fpd.2013.1551. Epub 2013 Sep 25.
Salmonella is one of the most important foodborne pathogens, which causes a huge economic burden worldwide. To detect Salmonella rapidly is very meaningful in preventing salmonellosis and decreasing economic losses. Currently, isolation of Salmonella is confirmed by biochemical and serobased serotyping methods, which are time consuming, labor intensive, and complicated. To solve this problem, a hexa-plex polymerase chain reaction (PCR) method was developed using comparative genomics analysis and multiplex PCR technology to detect Salmonella and Salmonella Typhimurium, Salmonella Enteritidis, Salmonella Agona, Salmonella Choleraesuis, and Salmonella Pullorum simultaneously. The accuracy of this method was tested by a collection of 142 Salmonella. Furthermore, the strategy described in this article to mine serovar-specific fragments for Salmonella could be used to find specific fragments for other Salmonella serotypes and bacteria. The combination of this strategy and multiplex PCR is promising in the rapid identification of foodborne pathogens.
沙门氏菌是最重要的食源性致病菌之一，它在全球范围内造成了巨大的经济负担。快速检测沙门氏菌对于预防沙门氏菌病和减少经济损失具有重要意义。目前，沙门氏菌的分离鉴定依赖于生化和血清学的血清分型方法，这些方法耗时、费力且复杂。为了解决这个问题，我们使用比较基因组学分析和多重 PCR 技术，开发了一种六重聚合酶链反应（PCR）方法，用于同时检测沙门氏菌和鼠伤寒沙门氏菌、肠炎沙门氏菌、肠炎沙门氏菌、肠炎沙门氏菌、肠炎沙门氏菌和肠炎沙门氏菌。通过收集的 142 株沙门氏菌对该方法的准确性进行了测试。此外，本文中描述的用于挖掘沙门氏菌血清型特异性片段的策略可用于寻找其他沙门氏菌血清型和细菌的特异性片段。该策略与多重 PCR 的结合有望用于快速鉴定食源性病原体。
