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在埃及索哈杰市,使用属于该基因的属特异性引物检测鸡尸体中的物种。

Detection of species in chicken carcasses using genus specific primer belong to gene in Sohag city, Egypt.

作者信息

Abdel-Aziz Nahed Mahmoud

机构信息

Department of Food Hygiene, Faculty of Veterinary Medicine, Sohag University, Naser Street, Sohag, Egypt.

出版信息

Vet World. 2016 Oct;9(10):1125-1128. doi: 10.14202/vetworld.2016.1125-1128. Epub 2016 Oct 22.

Abstract

AIM

This study aimed to detect species found as contaminants in chicken carcass (thigh, breast, wings, liver, and gizzard).

MATERIALS AND METHODS

A total of 75 chicken samples including thigh, breast, wings, liver, and gizzard (15 of each) were collected from different markets in Sohag city for detection of species by culture methods, biochemical tests, serology, and polymerase chain reaction.

RESULTS

The overall incidence of contamination of 75 examined samples was found to be 6.6% with the higher percentage of being isolated from liver samples (13.3%) followed by thigh, wings, gizzard (6.6%) while breast show negative result.

CONCLUSION

Results in this study indicate that contamination of chicken carcass with needs strict hygienic measures to prevent their transmission to human.

摘要

目的

本研究旨在检测在鸡胴体(大腿、胸脯、翅膀、肝脏和砂囊)中作为污染物发现的[某种微生物]种类。

材料与方法

从索哈杰市不同市场收集了总共75份鸡样本,包括大腿、胸脯、翅膀、肝脏和砂囊(各15份),通过培养方法、生化试验、血清学和聚合酶链反应检测[某种微生物]种类。

结果

在75份检测样本中,[某种微生物]污染的总体发生率为6.6%,其中从肝脏样本中分离出的比例较高(13.3%),其次是大腿、翅膀、砂囊(6.6%),而胸脯样本呈阴性结果。

结论

本研究结果表明,鸡胴体被[某种微生物]污染需要严格的卫生措施以防止其传播给人类。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69de/5104722/b561c4216d40/VetWorld-9-1125-g001.jpg

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