Wang Fei, Cao Yi, Li Fang, Shan Jianlin, Wen Tianlin
Department of Orthopaedic Surgery, The General Hospital of Beijing Military Area Command, No. 5 Nanmencang Road, Dongcheng District, Beijing 100700, China.
Department of Endocrinology, The General Hospital of Beijing Military Area Command, No. 5 Nanmencang Road, Dongcheng District, Beijing 100700, China.
Biomed Res Int. 2014;2014:320828. doi: 10.1155/2014/320828. Epub 2014 Dec 15.
Several studies report that the OPG is an important candidate gene in the pathogenesis of osteoporosis. This study aimed to detect the potential association of OPG gene polymorphisms with osteoporosis in postmenopausal women. We recruited 928 subjects containing 463 with primary postmenopausal osteoporosis and 465 healthy volunteers as controls. The BMD of neck hip, lumbar spine (L(2-4)), and total hip were assessed by dual-energy X-ray absorptiometry (DEXA). Through the created restriction site-polymerase chain reaction (CRS-PCR), PCR-restriction fragment length polymorphism (PCR-RFLP), and DNA sequencing methods, the g.18873C>T and g.27522G>A have been investigated. As for g.18873C>T, our data indicated that subjects with CC genotype have significantly higher BMD value than those of CT and TT genotypes (all P values < 0.05). As for g.27522G>A, the BMD values of subjects with GG genotype were significantly higher than those of GA and AA genotypes (all P values < 0.05). Our findings suggest that the OPG g.18873C>T and g.27522G>A genetic polymorphisms are associated with the decreased risk for osteoporosis in Chinese postmenopausal women.
多项研究报告称，骨保护素（OPG）是骨质疏松症发病机制中的一个重要候选基因。本研究旨在检测OPG基因多态性与绝经后女性骨质疏松症之间的潜在关联。我们招募了928名受试者，其中包括463名原发性绝经后骨质疏松症患者和465名健康志愿者作为对照。采用双能X线吸收法（DEXA）评估颈部、髋部、腰椎（L2 - 4）和全髋部的骨密度。通过创建限制酶切位点 - 聚合酶链反应（CRS - PCR）、聚合酶链反应 - 限制性片段长度多态性（PCR - RFLP）和DNA测序方法，对g.18873C>T和g.27522G>A进行了研究。对于g.18873C>T，我们的数据表明，CC基因型受试者的骨密度值显著高于CT和TT基因型受试者（所有P值<0.05）。对于g.27522G>A，GG基因型受试者的骨密度值显著高于GA和AA基因型受试者（所有P值<0.05）。我们的研究结果表明，OPG基因的g.18873C>T和g.27522G>A多态性与中国绝经后女性骨质疏松症风险降低有关。
