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适用于卵形疟原虫柯蒂斯亚种和卵形疟原虫沃里克里亚种鉴定及基因分型的遗传标记。

Genetic marker suitable for identification and genotyping of Plasmodium ovale curtisi and Plasmodium ovale wallikeri.

作者信息

Tanomsing Naowarat, Imwong Mallika, Sutherland Colin J, Dolecek Christiane, Hien Tran Tinh, Nosten Francois, Day Nicholas P J, White Nicholas J, Snounou Georges

机构信息

Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

出版信息

J Clin Microbiol. 2013 Dec;51(12):4213-6. doi: 10.1128/JCM.01527-13. Epub 2013 Sep 25.

DOI:10.1128/JCM.01527-13
PMID:24068009
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3838052/
Abstract

We present a seminested PCR method that specifically discriminates between Plasmodium ovale curtisi and P. ovale wallikeri with high sensitivity. The test is based on species-specific amplification of a size-polymorphic fragment of the tryptophan-rich antigen gene, potra, which also permits discrimination of intraspecific sequence variants at this locus.

摘要

我们提出了一种半巢式聚合酶链反应（PCR）方法，该方法能够以高灵敏度特异性地区分柯氏疟原虫和沃氏疟原虫。该检测基于对富含色氨酸抗原基因potra的一个大小多态性片段进行种特异性扩增，这也能够区分该位点的种内序列变异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c57b/3838052/8f2044a1e418/zjm9990929740002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c57b/3838052/28271c7501bf/zjm9990929740001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c57b/3838052/8f2044a1e418/zjm9990929740002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c57b/3838052/28271c7501bf/zjm9990929740001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c57b/3838052/8f2044a1e418/zjm9990929740002.jpg

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