利用Vitek基质辅助激光解吸电离飞行时间质谱法从固体培养基中鉴定分枝杆菌的热灭活和细胞破碎方案比较
Comparison of heat inactivation and cell disruption protocols for identification of mycobacteria from solid culture media by use of vitek matrix-assisted laser desorption ionization-time of flight mass spectrometry.
作者信息
Machen Alexandra, Kobayashi Miwako, Connelly Mary Robin, Wang Yun F Wayne
机构信息
Emory University School of Medicine.
出版信息
J Clin Microbiol. 2013 Dec;51(12):4226-9. doi: 10.1128/JCM.02612-13. Epub 2013 Sep 25.
Abstract
Two novel protocols for inactivation and extraction were developed and used to identify 107 Mycobacterium clinical isolates, including Mycobacterium tuberculosis complex, from solid cultures using Vitek matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. The protocol using heat inactivation with sonication and cell disruption with glass beads resulted in 82.2% and 88.8% species and genus level identifications, respectively.
摘要
开发了两种用于灭活和提取的新方案，并将其用于从固体培养物中鉴定107株分枝杆菌临床分离株，包括结核分枝杆菌复合群，采用Vitek基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱法。使用热灭活结合超声处理以及玻璃珠细胞破碎的方案，分别在种水平和属水平上实现了82.2%和88.8%的鉴定率。
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