在黑热病后皮肤利什曼病的斑疹、丘疹及混合性丘疹 - 结节性皮损诊断中，对皮肤活检标本进行聚合酶链反应（PCR）和印片涂片显微镜分析的比较评估。
Comparative evaluation of PCR and imprint smear microscopy analyses of skin biopsy specimens in diagnosis of macular, papular, and mixed papulo-nodular lesions of post-kala-azar dermal leishmaniasis.
作者信息
Verma Neena, Singh Dharmendra, Pandey Krishna, Das Vidya Nand Rabi, Lal Chandra Shekhar, Verma Rakesh Bihari, Sinha Prabhat Kumar, Das Pradeep
机构信息
Department of Pathology.
出版信息
J Clin Microbiol. 2013 Dec;51(12):4217-9. doi: 10.1128/JCM.01482-13. Epub 2013 Sep 25.
Abstract
Diagnosis of post-kala-azar dermal leishmaniasis (PKDL), particularly the macular form, is difficult when based on microscopy. This study compared the results of nested PCR (91.9% positive samples) with imprint smear microscopy (70.9% positive samples) for 62 PKDL samples. We found that nested PCR, which indicated 87.5% positivity for the macular lesions, compared to 41.6% positivity by imprint smear microscopy, is an efficient method for early diagnosis of PKDL.
摘要
内脏利什曼病后皮肤利什曼病（PKDL），尤其是斑疹型的诊断，基于显微镜检查时存在困难。本研究比较了62份PKDL样本的巢式PCR（阳性样本91.9%）与印片涂片显微镜检查（阳性样本70.9%）的结果。我们发现，巢式PCR对斑疹性病变的阳性率为87.5%，而印片涂片显微镜检查的阳性率为41.6%，巢式PCR是PKDL早期诊断的有效方法。
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