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本文引用的文献

1
Parasite detection in patients with post kala-azar dermal leishmaniasis in India: a comparison between molecular and immunological methods.印度黑热病后皮肤利什曼病患者的寄生虫检测：分子方法与免疫方法的比较
J Clin Pathol. 2003 Nov;56(11):840-3. doi: 10.1136/jcp.56.11.840.
2
Post-kala-azar dermal leishmaniasis associated with AIDS.与艾滋病相关的黑热病后皮肤利什曼病
Braz J Infect Dis. 2002 Dec;6(6):313-6. doi: 10.1590/s1413-86702002000600008. Epub 2003 Nov 3.
3
Post-kala-azar dermal leishmaniasis.黑热病后皮肤利什曼病
Lancet Infect Dis. 2003 Feb;3(2):87-98. doi: 10.1016/s1473-3099(03)00517-6.
4
Development of a species-specific PCR assay for detection of Leishmania donovani in clinical samples from patients with kala-azar and post-kala-azar dermal leishmaniasis.开发一种用于检测黑热病和黑热病后皮肤利什曼病患者临床样本中杜氏利什曼原虫的种特异性聚合酶链反应检测方法。
J Clin Microbiol. 2001 Mar;39(3):849-54. doi: 10.1128/JCM.39.3.849-854.2001.
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Use of PCR for diagnosis of post-kala-azar dermal leishmaniasis.聚合酶链反应用于诊断黑热病后皮肤利什曼病。
J Clin Microbiol. 1998 Jun;36(6):1621-4. doi: 10.1128/JCM.36.6.1621-1624.1998.
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Post-kala-azar dermal Leishmaniasis in an HIV-patient.一名艾滋病患者的黑热病后皮肤利什曼病
Int J Dermatol. 1996 Apr;35(4):303-4. doi: 10.1111/j.1365-4362.1996.tb03014.x.
7
Enzymatic amplification of mini-exon-derived RNA gene spacers of Leishmania donovani: primers and probes for DNA diagnosis.杜氏利什曼原虫小外显子衍生RNA基因间隔区的酶促扩增：用于DNA诊断的引物和探针
Parasitology. 1993 Dec;107 ( Pt 5):509-17. doi: 10.1017/s0031182000068086.
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Post-kala-azar dermal leishmaniasis: a light and electron microscopic study of 18 cases.黑热病后皮肤利什曼病：18例病例的光镜和电镜研究
J Cutan Pathol. 1993 Aug;20(4):320-5. doi: 10.1111/j.1600-0560.1993.tb01269.x.
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Development and application of the polymerase chain reaction for the detection and identification of Leishmania parasites in clinical material.用于检测和鉴定临床样本中利什曼原虫寄生虫的聚合酶链反应的开发与应用。
Arch Inst Pasteur Tunis. 1993 Jul-Oct;70(3-4):419-31.
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Post-kala-azar dermal leishmaniasis.黑热病后皮肤利什曼病
Int J Dermatol. 1995 Feb;34(2):85-91. doi: 10.1111/j.1365-4362.1995.tb03584.x.

用于检测黑热病后皮肤利什曼病病变部位皮肤刮取物中杜氏利什曼原虫的巢式聚合酶链反应检测法。

Nested PCR assay for detection of Leishmania donovani in slit aspirates from post-kala-azar dermal Leishmaniasis Lesions.

作者信息

Sreenivas Gannavaram, Ansari N A, Kataria Joginder, Salotra Poonam

机构信息

Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi, India.

出版信息

J Clin Microbiol. 2004 Apr;42(4):1777-8. doi: 10.1128/JCM.42.4.1777-1778.2004.

DOI:10.1128/JCM.42.4.1777-1778.2004
PMID:15071047
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC387583/
Abstract

A nested PCR assay to detect parasite DNA in slit aspirates from skin lesions of patients with post-kala-azar dermal lesihmaniasis (PKDL) is described. PCR results were positive in 27 of 29 (93%) samples by nested PCR assay, while only 20 of 29 (69%) were positive in a primary PCR assay. The nested PCR assay allowed reliable diagnosis of PKDL in a noninvasive manner.

摘要

本文描述了一种巢式PCR检测方法，用于检测黑热病后皮肤利什曼病（PKDL）患者皮肤病变刮片中的寄生虫DNA。通过巢式PCR检测，29份样本中有27份（93%）结果呈阳性，而在初次PCR检测中，29份样本中只有20份（69%）呈阳性。巢式PCR检测能够以非侵入性方式对PKDL进行可靠诊断。