用于检测黑热病后皮肤利什曼病病变部位皮肤刮取物中杜氏利什曼原虫的巢式聚合酶链反应检测法。
Nested PCR assay for detection of Leishmania donovani in slit aspirates from post-kala-azar dermal Leishmaniasis Lesions.
作者信息
Sreenivas Gannavaram, Ansari N A, Kataria Joginder, Salotra Poonam
机构信息
Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi, India.
出版信息
J Clin Microbiol. 2004 Apr;42(4):1777-8. doi: 10.1128/JCM.42.4.1777-1778.2004.
Abstract
A nested PCR assay to detect parasite DNA in slit aspirates from skin lesions of patients with post-kala-azar dermal lesihmaniasis (PKDL) is described. PCR results were positive in 27 of 29 (93%) samples by nested PCR assay, while only 20 of 29 (69%) were positive in a primary PCR assay. The nested PCR assay allowed reliable diagnosis of PKDL in a noninvasive manner.
摘要
本文描述了一种巢式PCR检测方法，用于检测黑热病后皮肤利什曼病（PKDL）患者皮肤病变刮片中的寄生虫DNA。通过巢式PCR检测，29份样本中有27份（93%）结果呈阳性，而在初次PCR检测中，29份样本中只有20份（69%）呈阳性。巢式PCR检测能够以非侵入性方式对PKDL进行可靠诊断。
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