Department of Veterinary Teaching Hospital, School of Veterinary Medicine, Kitasato University, Towada, Aomori, 034-8628, Japan.
Mol Biol Rep. 2013 Dec;40(12):6747-55. doi: 10.1007/s11033-013-2791-0. Epub 2013 Sep 26.
The common marmoset (Callithrix jacchus) is increasingly being used as a non-human primate animal model in biomedical research. To perform accurate quantitative analysis of gene expression by quantitative reverse transcription polymerase chain reaction, reliable reference genes should be selected. In this study, we evaluated the expressions of 11 widely used reference genes: ACTB, ATP5F1, B2M, GAPDH, HPRT1, PGK1, PPIA, RN18S1, RPLP0, TBP and UBC in 12 tissues and five brain areas of healthy common marmosets. NormFinder and geNorm indicated that the most suitable reference genes for cross-sectional studies of the 17 tissues were RN18S1 and RPLP0. Conversely, ACTB and PPIA were the most suitable for analyzing brain samples; however, the expression of PGK1 fluctuated among brain areas. These results indicate that suitable reference genes differ between the tissues examined. This study provides fundamental information for gene expression studies of the common marmoset and highlights the importance of validating reference genes before quantification of target mRNAs.
普通绒猴（Callithrix jacchus）越来越多地被用作生物医学研究中的非人类灵长类动物模型。为了通过定量逆转录聚合酶链反应进行准确的基因表达定量分析，应选择可靠的参考基因。在这项研究中，我们评估了 11 种广泛使用的参考基因在 12 种组织和 5 个健康普通绒猴大脑区域中的表达：ACTB、ATP5F1、B2M、GAPDH、HPRT1、PGK1、PPIA、RN18S1、RPLP0、TBP 和 UBC。NormFinder 和 geNorm 表明，用于 17 种组织的横截面研究的最适参考基因是 RN18S1 和 RPLP0。相反，ACTB 和 PPIA 是分析脑样本的最适基因；然而，PGK1 在脑区之间的表达波动较大。这些结果表明，所检查的组织之间适合的参考基因不同。本研究为普通绒猴的基因表达研究提供了基础信息，并强调了在定量分析靶 mRNA 之前验证参考基因的重要性。
