Cáceres Manuel, Gentina Juan C, Aroca Germán
School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2147, Valparaíso, Chile.
Biotechnol Lett. 2014 Jan;36(1):69-74. doi: 10.1007/s10529-013-1339-7. Epub 2013 Sep 26.
Oxidation of methane by methanotrophs, Methylomicrobium album and Methylocystis sp., was measured at several initial concentrations of H2S and NH3 in the headspace of stoppered flasks, at the same initial concentration of methane as sole carbon and energy source: 15 % (v/v). No effect was observed at 0.01 % (v/v) H2S and 0.025 % (v/v) NH3 in gas phase but over 0.05 and 0.025 % (v/v), respectively, they inhibited the oxidation of methane. The effect of H2S was stronger in Methylocystis sp. and both microorganisms were similarly affected by NH3. Depending on their concentrations in gas phase, H2S and NH3 can thus affect the rate of oxidation of methane and biomass growth of both methanotrophs.
在封闭烧瓶的顶空中，在几种初始硫化氢（H₂S）和氨（NH₃）浓度下，以甲烷作为唯一碳源和能源，初始浓度为15%（v/v），测定了甲烷氧化菌——白色甲基微菌（Methylomicrobium album）和甲基孢囊菌属（Methylocystis sp.）对甲烷的氧化作用。在气相中硫化氢浓度为0.01%（v/v）和氨浓度为0.025%（v/v）时未观察到影响，但当硫化氢浓度超过0.05%（v/v）和氨浓度超过0.025%（v/v）时，它们分别抑制了甲烷的氧化。硫化氢对甲基孢囊菌属的影响更强，并且两种微生物受氨的影响相似。因此，根据它们在气相中的浓度，硫化氢和氨可以影响甲烷氧化速率以及两种甲烷氧化菌的生物量增长。
