Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
World J Microbiol Biotechnol. 2014 Feb;30(2):669-80. doi: 10.1007/s11274-013-1489-8. Epub 2013 Sep 26.
This study was aimed to investigate the impact of subacute ruminal acidosis (SARA) on the diversity of liquid (LAB) and solid-associated bacteria (SAB) following high-grain feeding. Six ruminally cannulated goats were divided into two groups: one group was fed a hay diet (COD), and the other group was fed a high grain diet (SAID). Rumen liquids and rumen solids were sampled after 2 weeks adaption. SARA was diagnosed with a pH below 5.8 for 8 h. SAID decreased ruminal pH (P < 0.001) and increased the acetate (P = 0.017), propionate (P = 0.001), butyrate (P < 0.001) and total volatile fatty acid (P < 0.001) concentration in rumen compared with the COD. Denaturing gradient gel electrophoresis fingerprints analysis revealed a clear separation between both the diet and the fraction of rumen digesta in bacterial communities. Pyrosequencing analysis showed that the proportion of phylum Bacteroidetes in the SAID-LAB and SAID-SAB communities was less than in the COD group, whereas the SAID group had a greater percentage of Firmicutes in both the LAB and SAB libraries. UniFrac analyses and a Venn diagram revealed a large difference between the two diets in the diversity of rumen bacterial communities. Overall, our findings revealed that SARA feeding did alter the community structure of rumen liquids and rumen solids. Thus, manipulation of dietary factors, such as ratio of forage to concentrate may have the potential to alter the microbial composition of rumen liquid and rumen solid.
本研究旨在探究高精谷物饲养后亚急性瘤胃酸中毒（SARA）对液体（LAB）和固体相关细菌（SAB）多样性的影响。6 只瘤胃内置有瘘管的山羊被分为两组：一组喂食干草日粮（COD），另一组喂食高谷物日粮（SAID）。适应 2 周后采集瘤胃液和瘤胃固体样本。当 pH 值低于 5.8 且持续 8 小时时诊断为 SARA。与 COD 相比，SAID 降低了瘤胃 pH 值（P < 0.001），增加了乙酸（P = 0.017）、丙酸（P = 0.001）、丁酸（P < 0.001）和总挥发性脂肪酸（P < 0.001）浓度。变性梯度凝胶电泳指纹图谱分析显示，两种日粮和瘤胃消化物的细菌群落之间存在明显的分离。焦磷酸测序分析显示，SAID-LAB 和 SAID-SAB 群落中厚壁菌门的比例小于 COD 组，而 SAID 组在 LAB 和 SAB 文库中均有更多的Firmicutes。UniFrac 分析和 Venn 图显示，两种日粮在瘤胃细菌群落的多样性上有很大差异。总的来说，我们的研究结果表明，SARA 饲养确实改变了瘤胃液体和固体的群落结构。因此，对日粮因素（如粗饲料与精饲料的比例）的操纵可能具有改变瘤胃液体和固体微生物组成的潜力。
