Onderdonk A B, Cisneros R L, Finberg R, Crabb J H, Kasper D L
Infectious Diseases Research Laboratory, Tufts University School of Medicine, Boston, Massachusetts.
Rev Infect Dis. 1990 Jan-Feb;12 Suppl 2:S169-77. doi: 10.1093/clinids/12.supplement_2.s169.
Experimental animal model systems have been used by many investigators to explore the pathogenicity of obligate anaerobes. During the last 15 years, research in our laboratory has utilized an experimental model for intraabdominal sepsis to define the contribution of obligate anaerobes to the infectious process. These studies have shown that obligate anaerobes are important components of the polymicrobic flora present during such infection. Moreover, certain anaerobes, such as Bacteroides fragilis, possess specific virulence factors, such as the capsular polysaccharide, that appear to be important to the infectious process. More recent research has used modifications of the original model system to evaluate the host immune response to B. fragilis. These studies indicate that immunization with the capsular polysaccharide provides a T cell-dependent immunity to abscess development when animals are challenged with B. fragilis. It has also been shown that the killing of B. fragilis is T cell dependent. The observations made with regard to B. fragilis in this animal model system are discussed.
许多研究人员利用实验动物模型系统来探究专性厌氧菌的致病性。在过去15年中，我们实验室的研究利用腹腔内脓毒症的实验模型来确定专性厌氧菌在感染过程中的作用。这些研究表明，专性厌氧菌是此类感染期间存在的多种微生物菌群的重要组成部分。此外，某些厌氧菌，如脆弱拟杆菌，具有特定的毒力因子，如荚膜多糖，这似乎对感染过程很重要。最近的研究对原始模型系统进行了改进，以评估宿主对脆弱拟杆菌的免疫反应。这些研究表明，当用脆弱拟杆菌攻击动物时，用荚膜多糖免疫可提供对脓肿形成的T细胞依赖性免疫。还表明对脆弱拟杆菌的杀伤是T细胞依赖性的。本文讨论了在该动物模型系统中关于脆弱拟杆菌的观察结果。
