Hennig B
Eur J Biochem. 1975 Jun 16;55(1):167-83. doi: 10.1111/j.1432-1033.1975.tb02149.x.
The structure of cytochrome c during mouse development is investigated. For this purpose the amino acid sequence of cytochrome c of the adult mouse had to be determined. The structure of cytochrome c of adult differentiated mouse cells differs in two amino acid residues from the known amino acid sequence of rabbit cytochrome c. No indication of different forms of cytochrome c in the adult differentiated cells was obtained. The structure of cytochrome c from 11.5-day-old mouse embryos is identical with that of adult mouse tissues. Since germ cells after meiotic division are the immediate precursors of a new individual, the structure of cytochrome c from sperm-containing mice testes was investigated. By means of chromatography of the cytochrome c and of peptide maps and amino acid analyses of its tryptic peptides, it is shown that mouse testis contains two isocytochromes c in about equal amount. The structure of one of these two isocytochromes c is identical with the structure of the adult-type cytochrome c of mouse. The testis-specific cytochrome c, which is assumed to be located in the sperm cells, differs in 13 of its 104 amino acid residues from the adult-type cytochrome c. From comparison of the primary and the spatial structures of the adult-type and the sperm-type isocytochromes c with the known structures of cytochrome c of more than 65 different species it is concluded that the duplication of the cytochrome c structural gene, causing the existence of the two ontogenetic-specific isocytochromes c in mouse, has occurred early in the evolution of eucaryotes.
对小鼠发育过程中细胞色素c的结构进行了研究。为此，必须确定成年小鼠细胞色素c的氨基酸序列。成年分化小鼠细胞的细胞色素c结构与已知的兔细胞色素c氨基酸序列在两个氨基酸残基上有所不同。在成年分化细胞中未发现细胞色素c存在不同形式的迹象。11.5天大的小鼠胚胎的细胞色素c结构与成年小鼠组织的相同。由于减数分裂后的生殖细胞是新个体的直接前体，因此研究了含精子的小鼠睾丸中细胞色素c的结构。通过对细胞色素c进行色谱分析、对其胰蛋白酶肽段进行肽图分析和氨基酸分析，结果表明小鼠睾丸中含有两种等量的异细胞色素c。这两种异细胞色素c中的一种结构与成年型小鼠细胞色素c的结构相同。假定位于精子细胞中的睾丸特异性细胞色素c，在其104个氨基酸残基中有13个与成年型细胞色素c不同。通过将成年型和精子型异细胞色素c的一级结构和空间结构与65多种不同物种的细胞色素c已知结构进行比较，得出结论：细胞色素c结构基因的复制导致小鼠中存在两种个体发育特异性异细胞色素c，这一事件发生在真核生物进化的早期。
