Centre de Recherche en Automatique de Nancy (CRAN), Université de Lorraine , UMR 7039, Campus Sciences, BP 70239, 54506 Vandoeuvre-lès-Nancy Cedex, France.
ACS Nano. 2013 Oct 22;7(10):8645-57. doi: 10.1021/nn402792a. Epub 2013 Sep 26.
Near-infrared (NIR) imaging of the lymphatic system offers a sensitive, versatile, and accurate lymph node mapping to locate the first, potentially metastatic, draining nodes in the operating room. Many luminescent nanoprobes have received great attention in this field, and the design of nontoxic and bright nanosystems is of crucial importance. Fluorescent NIR-emitting dye doped silica nanoparticles represent valuable platforms to fulfill these scopes, providing sufficient brightness, resistance to photobleaching, and hydrophilic nontoxic materials. Here, we synthesized these highly stable core-shell nanoparticles with a programmable surface charge positioning and determined the effect of these physicochemical properties on their in vivo behavior. In addition, we characterized their fluorescence kinetic profile in the right axillary lymph node (RALN) mapping. We found that nanoparticles with negative charges hidden by a PEG shell are more appropriate than those with external negative charges in the mapping of lymph nodes. We also demonstrated the efficient excretion of these nanostructures by the hepatobiliary route and their nontoxicity in mice up to 3 months postinjection. These results indicate the potential future development of these fluorescent nanosystems for LN mapping.
近红外 (NIR) 成像技术可用于淋巴系统，能够灵敏、全面、准确地对淋巴结进行定位，从而在手术室中找到最初可能发生转移的引流淋巴结。在该领域，许多发光纳米探针受到了广泛关注，而设计无毒且明亮的纳米系统至关重要。荧光近红外发射染料掺杂二氧化硅纳米颗粒是实现这些目标的有价值的平台，提供了足够的亮度、抗光漂白性和亲水性无毒材料。在这里，我们合成了这些具有可编程表面电荷定位的高度稳定的核壳纳米颗粒，并确定了这些物理化学性质对其体内行为的影响。此外，我们还对其在右腋窝淋巴结 (RALN) 定位中的荧光动力学特性进行了表征。我们发现，带有隐藏在 PEG 壳内的负电荷的纳米颗粒比带有外部负电荷的纳米颗粒更适合用于淋巴结定位。我们还证明了这些纳米结构通过肝胆途径的有效排泄及其在注射后长达 3 个月的时间内对小鼠的非毒性。这些结果表明，这些荧光纳米系统具有用于 LN 定位的潜在未来发展前景。
