Humphrey D M
Department of Pathology, State University of New York, Syracuse 13210.
Am J Pathol. 1990 Feb;136(2):467-77.
Previously identified as a platelet-activating factor, 1-0-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine recently has been described as an inflammatory mediator with vasoactive and leukotactic properties. Histologic studies suggested that the local microvascular effects of this potent acetylated alkyl phosphoglyceride were limited in extent. Also, cytologic characterization of exudates was incomplete in tissue sections. Therefore, skin window chambers were used as an alternate model in which to explore the ability of the active form of this lipid mediator to diffuse in tissue. In addition, skin windows provided a convenient means to characterize the leukocytic exudates. Based on measurements of agonist-induced plasma exudation, the effect of 1-0-hexadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine appeared to be limited to the superficial microvasculature underlying the skin window surface. This interpretation was supported by a brief histologic study that revealed vascular labeling by colloidal carbon mostly in a narrow 150-microns dermal zone beneath chambers containing the phospholipid agonist. Finally, the leukocytic exudate recovered at 3 hours consisted of neutrophils and a small number of eosinophils. Thus the skin window model was useful to further characterize the leukocytic exudate, and it suggested that the potent vasoactive effects of the acetylated alkyl phosphoglyceride were limited by local conditions in tissue so as to produce a highly focused inflammatory response.
1-0-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱曾被鉴定为血小板激活因子，最近被描述为一种具有血管活性和白细胞趋化特性的炎症介质。组织学研究表明，这种强效乙酰化烷基磷酸甘油酯的局部微血管效应范围有限。此外，组织切片中渗出物的细胞学特征并不完整。因此，皮肤窗小室被用作一种替代模型，用以探究这种脂质介质的活性形式在组织中扩散的能力。此外，皮肤窗提供了一种方便的方法来表征白细胞渗出物。基于对激动剂诱导的血浆渗出的测量，1-0-十六烷基-2-乙酰基-sn-甘油-3-磷酸胆碱的作用似乎仅限于皮肤窗表面下方的浅表微血管。一项简短的组织学研究支持了这一解释，该研究显示胶体碳在含有磷脂激动剂的小室下方狭窄的150微米真皮区域中主要标记血管。最后，在3小时时回收的白细胞渗出物由中性粒细胞和少量嗜酸性粒细胞组成。因此，皮肤窗模型有助于进一步表征白细胞渗出物，并且表明乙酰化烷基磷酸甘油酯的强效血管活性作用受到组织局部条件的限制，从而产生高度集中的炎症反应。
